exRNAdisease

Entry Detail

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General Information

Database ID: exR0373052 RNA Name: FGD5-AS1 RNA Type: lncRNA Chromosome: chr3 Starnd: - Coordinate: Start Site(bp): 14920347 End Site(bp): 14948424 External Links: ENSG00000225733

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ACOT7 chr1 6264269 6394391 - ADAM10 chr15 58588809 58749791 - AGAP3 chr7 151085831 151144436 + AGGF1 chr5 77029251 77065234 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-3611 chr10 35079609 35079629 - hsa-miR-302c-3p chr4 112648366 112648388 - hsa-miR-421 chrX 74218392 74218414 - hsa-miR-1179 chr15 88608121 88608141 + hsa-miR-20a-5p chr13 91351072 91351094 + hsa-miR-493-5p chr14 100869075 100869096 + hsa-miR-497-5p chr17 7017979 7017999 - hsa-miR-105-5p chrX 152394458 152394480 - hsa-miR-520a-3p chr19 53690933 53690954 + hsa-miR-548o-3p chr20 38516607 38516628 + hsa-miR-196a-5p chr17 48632532 48632553 - hsa-miR-506-3p chrX 147230753 147230773 - hsa-miR-3622b-5p chr8 27701726 27701745 - hsa-miR-3681-5p chr2 12199138 12199159 + hsa-miR-126-5p chr9 136670616 136670636 + hsa-miR-892c-5p chrX 145992796 145992816 - hsa-miR-382-5p chr14 101054316 101054337 + hsa-miR-577 chr4 114656774 114656794 + hsa-miR-670-3p chr11 43559714 43559734 + hsa-miR-512-3p chr19 53666729 53666750 + hsa-miR-33a-5p chr22 41900949 41900969 + hsa-miR-424-5p chrX 134546680 134546701 - hsa-miR-3146 chr7 19705366 19705387 - hsa-miR-888-5p chrX 145994830 145994850 - hsa-miR-520c-3p chr19 53707506 53707527 + hsa-miR-15a-5p chr13 50049167 50049188 - hsa-miR-103a-3p chr5 168560904 168560926 - hsa-miR-520d-5p chr19 53720111 53720130 + hsa-miR-7853-5p chr6 6169380 6169400 - hsa-miR-1323 chr19 53671978 53671999 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC018521.1 chr17 47945424 47981736 + AC092902.2 chr3 125774714 125797953 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 945 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.