exRNAdisease

Entry Detail

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General Information

Database ID: exR0373069 RNA Name: HCG18 RNA Type: lncRNA Chromosome: chr6 Starnd: - Coordinate: Start Site(bp): 30286690 End Site(bp): 30327382 External Links: ENSG00000231074

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AKT1S1 chr19 49869033 49878459 - AKT2 chr19 40230317 40285536 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-15b-5p chr3 160404607 160404628 + hsa-miR-545-5p chrX 74287164 74287185 - hsa-miR-324-5p chr17 7223343 7223364 - hsa-miR-4677-3p chr1 243346225 243346246 + hsa-miR-424-5p chrX 134546680 134546701 - hsa-miR-148b-3p chr12 54337278 54337299 + hsa-miR-4739 chr17 79707216 79707240 - hsa-miR-3174 chr15 90006764 90006786 + hsa-miR-195-5p chr17 7017667 7017687 - hsa-miR-148a-3p chr7 25949922 25949943 - hsa-miR-449c-5p chr5 55172313 55172337 - hsa-miR-449a chr5 55170586 55170607 - hsa-miR-449b-5p chr5 55170706 55170727 - hsa-miR-16-5p chr3 160404754 160404775 + hsa-miR-34a-5p chr1 9151735 9151756 - hsa-miR-4756-5p chr20 54068452 54068474 - hsa-miR-1321 chrX 85835832 85835849 + hsa-miR-499a-5p chr20 34990408 34990428 + hsa-miR-3614-5p chr17 56891319 56891341 - hsa-miR-495-3p chr14 101033804 101033825 + hsa-miR-3605-5p chr1 33332449 33332471 - hsa-miR-15a-5p chr13 50049167 50049188 - hsa-miR-5688 chr3 85385761 85385782 + hsa-miR-497-5p chr17 7017979 7017999 - hsa-miR-6838-5p chr7 44073407 44073428 - hsa-miR-4508 chr15 23562107 23562123 - hsa-miR-34b-5p chr11 111512950 111512972 + hsa-miR-2682-5p chr1 98045307 98045329 - hsa-miR-499b-5p chr20 34990443 34990463 - hsa-miR-34c-5p chr11 111513451 111513473 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AL022157.1 chrX 57121662 57127243 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 945 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.