exRNAdisease

Entry Detail

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General Information

Database ID: exR0373087 RNA Name: KCNQ1OT1 RNA Type: lncRNA Chromosome: chr11 Starnd: - Coordinate: Start Site(bp): 2608328 End Site(bp): 2699994 External Links: ENSG00000269821

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ADAT1 chr16 75596981 75623300 - AL355315.1 chr10 97584374 97673910 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-4640-5p chr6 30890891 30890913 + hsa-miR-196b-5p chr7 27169528 27169549 - hsa-miR-378d chr4 5923279 5923298 - hsa-miR-129-2-3p chr11 43581450 43581471 + hsa-miR-98-5p chrX 53556299 53556320 - hsa-miR-4644 chr6 170330813 170330835 + hsa-let-7g-5p chr3 52268336 52268357 - hsa-miR-140-3p chr16 69933142 69933162 + hsa-miR-324-3p chr17 7223308 7223330 - hsa-miR-3918 chr6 158764715 158764735 - hsa-miR-1914-3p chr20 63941474 63941495 - hsa-miR-378c chr10 130962634 130962658 - hsa-let-7b-5p chr22 46113691 46113712 + hsa-miR-339-3p chr7 1022955 1022977 - hsa-miR-181a-5p chr1 198859108 198859130 - hsa-miR-214-5p chr1 172138857 172138878 - hsa-miR-605-3p chr10 51299623 51299644 + hsa-miR-513b-5p chrX 147199093 147199114 - hsa-miR-5581-3p chr1 37500935 37500956 - hsa-miR-124-3p chr8 64379210 64379231 + hsa-miR-2278 chr9 94809977 94809998 + hsa-miR-532-3p chrX 50003204 50003225 + hsa-miR-130b-3p chr22 21653354 21653375 + hsa-miR-145-5p chr5 149430661 149430683 + hsa-miR-4756-5p chr20 54068452 54068474 - hsa-miR-362-3p chrX 50009005 50009026 + hsa-miR-134-5p chr14 101054694 101054715 + hsa-miR-148a-3p chr7 25949922 25949943 - hsa-let-7i-5p chr12 62603691 62603712 + hsa-miR-4524b-5p chr17 69099570 69099590 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC005034.3 chr2 75697583 75697996 + AC010186.4 chr12 9642872 9657070 + AC026471.1 chr16 31456711 31459736 - AD000090.1 chr19 35557956 35581954 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 945 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.