exRNAdisease

Entry Detail

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General Information

Database ID: exR0373244 RNA Name: OIP5-AS1 RNA Type: lncRNA Chromosome: chr15 Starnd: + Coordinate: Start Site(bp): 41283990 End Site(bp): 41309737 External Links: ENSG00000247556

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ACTB chr7 5527148 5563784 - AGPAT1 chr6 32168212 32178096 - AC139530.2 chr17 81703371 81720539 + ACO1 chr9 32384603 32454769 + ACTR2 chr2 65227753 65271253 + AK3 chr9 4709556 4742043 - AL136295.5 chr14 24147548 24166452 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-641 chr19 40282603 40282626 - hsa-miR-320a-3p chr8 22244975 22244996 - hsa-miR-141-3p chr12 6964155 6964176 + hsa-miR-378a-3p chr5 149732867 149732888 + hsa-miR-137-3p chr1 98046091 98046113 - hsa-miR-28-5p chr3 188688794 188688815 + hsa-miR-552-3p chr1 34669614 34669634 - hsa-miR-3681-3p chr2 12199174 12199195 + hsa-miR-892c-3p chrX 145992760 145992781 - hsa-miR-1252-5p chr12 79419261 79419282 + hsa-miR-514b-5p chrX 147250197 147250218 - hsa-miR-1323 chr19 53671978 53671999 + hsa-miR-126-5p chr9 136670616 136670636 + hsa-miR-429 chr1 1169055 1169076 + hsa-miR-4524b-5p chr17 69099570 69099590 + hsa-miR-1270 chr19 20399320 20399342 - hsa-miR-32-5p chr9 109046272 109046293 - hsa-miR-378h chr5 154829466 154829486 + hsa-miR-448 chrX 114823524 114823545 + hsa-miR-29a-3p chr7 130876748 130876769 - hsa-miR-378i chr22 41923271 41923291 - hsa-miR-218-5p chr5 168768211 168768231 - hsa-miR-135a-5p chr12 97563834 97563856 + hsa-miR-217-5p chr2 55983020 55983042 - hsa-miR-548o-3p chr20 38516607 38516628 + hsa-miR-340-5p chr5 180015361 180015382 - hsa-miR-4524a-5p chr17 69099606 69099627 - hsa-miR-374b-3p chrX 74218557 74218578 - hsa-miR-422a chr15 63870989 63871010 - hsa-miR-579-3p chr5 32394392 32394414 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC079781.5 chr7 97851688 97972985 - AC106864.1 chr4 112693047 112706810 - AC108860.2 chr8 94637285 94639467 - AL022311.1 chr22 37876148 37895563 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 945 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.