exRNAdisease

Entry Detail

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General Information

Database ID: exR0373354 RNA Name: TUG1 RNA Type: lncRNA Chromosome: chr22 Starnd: + Coordinate: Start Site(bp): 30969245 End Site(bp): 30979395 External Links: ENSG00000253352

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AC005258.1 chr19 2269525 2341172 + ACOT7 chr1 6264269 6394391 - ADNP chr20 50888916 50931437 - AGO1 chr1 35869808 35930532 + AC090004.1 chr3 14135217 14148252 + ADH5 chr4 99070978 99088801 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-590-5p chr7 74191213 74191234 + hsa-miR-3681-3p chr2 12199174 12199195 + hsa-miR-7151-5p chr10 67403388 67403410 - hsa-miR-216a-3p chr2 55988981 55989002 - hsa-miR-9-3p chr15 89368071 89368092 + hsa-miR-362-5p chrX 50008968 50008991 + hsa-miR-299-3p chr14 101023832 101023853 + hsa-miR-145-5p chr5 149430661 149430683 + hsa-miR-4465 chr6 140683855 140683876 + hsa-miR-500b-5p chrX 50010682 50010699 + hsa-miR-4766-5p chr22 40813927 40813947 - hsa-miR-1294 chr5 154347153 154347174 + hsa-miR-425-5p chr3 49020199 49020221 - hsa-miR-495-3p chr14 101033804 101033825 + hsa-miR-499b-5p chr20 34990443 34990463 - hsa-miR-519e-5p chr19 53679953 53679974 + hsa-miR-3129-5p chr2 189133082 189133103 - hsa-miR-1277-5p chrX 118386401 118386424 + hsa-miR-153-3p chr2 219294126 219294147 - hsa-miR-509-3p chrX 147258775 147258796 - hsa-miR-9-5p chr5 88666902 88666924 - hsa-miR-511-3p chr10 17845160 17845179 + hsa-miR-382-5p chr14 101054316 101054337 + hsa-miR-542-3p chrX 134541364 134541385 - hsa-miR-27b-3p chr9 95085505 95085525 + hsa-miR-1343-3p chr11 34941888 34941909 + hsa-miR-221-3p chrX 45746180 45746202 - hsa-miR-376a-3p chr14 101040825 101040845 + hsa-miR-561-5p chr2 188297517 188297538 + hsa-miR-196b-5p chr7 27169528 27169549 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AD000090.1 chr19 35557956 35581954 + AL021707.1 chr22 38739003 38749041 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 945 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.