exRNAdisease

Entry Detail

---



General Information

Database ID: exR0373371 RNA Name: ZFAS1 RNA Type: lncRNA Chromosome: chr20 Starnd: + Coordinate: Start Site(bp): 49278178 End Site(bp): 49299600 External Links: ENSG00000177410

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ABHD14B chr3 51968510 51983409 - AC138811.2 chr16 18788063 18801519 - ACTG1 chr17 81509971 81523847 - AEN chr15 88621337 88632281 + AC107871.1 chr15 68184032 68229718 - AC134669.1 chr17 31305213 31318831 - AGTPBP1 chr9 85546539 85742029 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-96-5p chr7 129774739 129774761 - hsa-miR-7-5p chr19 4770700 4770723 + hsa-miR-150-5p chr19 49500832 49500853 - hsa-miR-193a-3p chr17 31560050 31560071 + hsa-miR-34b-5p chr11 111512950 111512972 + hsa-miR-432-5p chr14 100884496 100884518 + hsa-miR-193b-3p chr16 14304017 14304038 + hsa-miR-520a-5p chr19 53690895 53690915 + hsa-miR-525-5p chr19 53697547 53697567 + hsa-miR-510-5p chrX 147272378 147272399 - hsa-miR-642a-5p chr19 45674943 45674964 + hsa-miR-421 chrX 74218392 74218414 - hsa-miR-582-3p chr5 59703630 59703651 - hsa-miR-654-3p chr14 101040269 101040290 + hsa-miR-1323 chr19 53671978 53671999 + hsa-miR-1271-5p chr5 176367960 176367981 + hsa-miR-548o-3p chr20 38516607 38516628 + hsa-miR-449c-5p chr5 55172313 55172337 - hsa-miR-2682-5p chr1 98045307 98045329 - hsa-miR-3121-3p chr1 180438323 180438344 - hsa-miR-3924 chr10 57304489 57304510 - hsa-miR-3942-5p chr15 35372321 35372342 - hsa-miR-4640-3p chr6 30890949 30890970 + hsa-miR-4703-5p chr13 51552599 51552621 + hsa-miR-5047 chr17 64501230 64501250 - hsa-miR-6835-3p chr6 34240714 34240736 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC007952.4 chr17 19112000 19112636 - AC139149.1 chr17 81514047 81527776 + AC147651.4 chr7 603185 608482 + AC245014.3 chr1 145281116 145281462 + AL022311.1 chr22 37876148 37895563 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 945 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.