| Experiment ID | EXP00034 |
| Reference | Title: MicroRNA expression profiling of human breast cancer identifies new markers oftumor subtype. Author: Blenkiron C, Goldstein LD, Thorne NP, Spiteri I, Chin SF, Dunning MJ,Barbosa-Morais NL, Teschendorff AE, Green AR, Ellis IO, Tavaré S, Caldas C, MiskaEA. Journal: Genome Biol. 2007;8(10):R214. Abstract: BACKGROUND: MicroRNAs (miRNAs), a class of short non-coding RNAs found in manyplants and animals, often act post-transcriptionally to inhibit gene expression.RESULTS: Here we report the analysis of miRNA expression in 93 primary humanbreast tumors, using a bead-based flow cytometric miRNA expression profilingmethod. Of 309 human miRNAs assayed, we identify 133 miRNAs expressed in humanbreast and breast tumors. We used mRNA expression profiling to classify thebreast tumors as luminal A, luminal B, basal-like, HER2+ and normal-like. Anumber of miRNAs are differentially expressed between these molecular tumorsubtypes and individual miRNAs are associated with clinicopathological factors.Furthermore, we find that miRNAs could classify basal versus luminal tumorsubtypes in an independent data set. In some cases, changes in miRNA expressioncorrelate with genomic loss or gain; in others, changes in miRNA expression arelikely due to changes in primary transcription and or miRNA biogenesis. Finally, the expression of DICER1 and AGO2 is correlated with tumor subtype and mayexplain some of the changes in miRNA expression observed.CONCLUSION: This study represents the first integrated analysis of miRNAexpression, mRNA expression and genomic changes in human breast cancer and mayserve as a basis for functional studies of the role of miRNAs in the etiology of breast cancer. Furthermore, we demonstrate that bead-based flow cytometric miRNA expression profiling might be a suitable platform to classify breast cancer into prognostic molecular subtypes. PMID: 17922911 |
| Expressiion Profile | Description: MicroRNA expression profiling of human breast cancer identifies new markers of tumour subtype Organism: Homo sapiens GEO ID: GSE7842 Platform: GPL5173 Number of samples: 119 |
| Design and Sample | Cancer Type: breast cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: breast tumour Control Sample: normal breast Num of Case: 93 Num of Control: 5 Quantification Software: Limma Num of miRNAs: 137 |
| Identification | Num of Up: 10 Num of Down: 8 |