| Experiment ID | EXP00045 |
| Reference | Title: Genomic profiling of microRNA and messenger RNA reveals deregulated microRNAexpression in prostate cancer. Author: Ambs S, Prueitt RL, Yi M, Hudson RS, Howe TM, Petrocca F, Wallace TA, Liu CG, Volinia S, Calin GA, Yfantis HG, Stephens RM, Croce CM. Journal: Cancer Res. 2008 Aug 1;68(15):6162-70. doi: 10.1158/0008-5472.CAN-08-0144. Abstract: MicroRNAs are small noncoding RNAs that regulate the expression of protein-codinggenes. To evaluate the involvement of microRNAs in prostate cancer, we determinedgenome-wide expression of microRNAs and mRNAs in 60 primary prostate tumors and16 nontumor prostate tissues. The mRNA analysis revealed that key components ofmicroRNA processing and several microRNA host genes, e.g., MCM7 and C9orf5, were significantly up-regulated in prostate tumors. Consistent with these findings,tumors expressed the miR-106b-25 cluster, which maps to intron 13 of MCM7, andmiR-32, which maps to intron 14 of C9orf5, at significantly higher levels thannontumor prostate. The expression levels of other microRNAs, including a numberof miR-106b-25 cluster homologues, were also altered in prostate tumors.Additional differences in microRNA abundance were found between organ-confinedtumors and those with extraprostatic disease extension. Lastly, we found evidencethat some microRNAs are androgen-regulated and that tumor microRNAs influencetranscript abundance of protein-coding target genes in the cancerous prostate. Incell culture, E2F1 and p21/WAF1 were identified as targets of miR-106b, Bim ofmiR-32, and exportin-6 and protein tyrosine kinase 9 of miR-1. In summary,microRNA expression becomes altered with the development and progression ofprostate cancer. Some of these microRNAs regulate the expression ofcancer-related genes in prostate cancer cells. PMID: 18676839 |
| Expressiion Profile | Description: Genomic profiling of microRNA and messenger RNA reveals deregulated microRNA expression in prostate cancer Organism: Homo sapiens GEO ID: GSE8126 Platform: GPL5180 Number of samples: 76 |
| Design and Sample | Cancer Type: prostate cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: prostate adenocarcinoma pt stage 2 Control Sample: normal prostate Num of Case: 35 Num of Control: 16 Quantification Software: Limma Num of miRNAs: 310 |
| Identification | Num of Up: 14 Num of Down: 11 |