| Experiment ID | EXP00047 |
| Reference | Title: Characterization of global microRNA expression reveals oncogenic potential ofmiR-145 in metastatic colorectal cancer. Author: Arndt GM, Dossey L, Cullen LM, Lai A, Druker R, Eisbacher M, Zhang C, Tran N, Fan H, Retzlaff K, Bittner A, Raponi M. Journal: BMC Cancer. 2009 Oct 20;9:374. doi: 10.1186/1471-2407-9-374. Abstract: BACKGROUND: MicroRNAs (MiRNAs) are short non-coding RNAs that control proteinexpression through various mechanisms. Their altered expression has been shown tobe associated with various cancers. The aim of this study was to profile miRNAexpression in colorectal cancer (CRC) and to analyze the function of specificmiRNAs in CRC cells. MirVana miRNA Bioarrays were used to determine the miRNAexpression profile in eight CRC cell line models, 45 human CRC samples ofdifferent stages, and four matched normal colon tissue samples. SW620 CRC cellswere stably transduced with miR-143 or miR-145 expression vectors and analyzed invitro for cell proliferation, cell differentiation and anchorage-independentgrowth. Signalling pathways associated with differentially expressed miRNAs were identified using a gene set enrichment analysis.RESULTS: The expression analysis of clinical CRC samples identified 37 miRNAsthat were differentially expressed between CRC and normal tissue. Furthermore,several of these miRNAs were associated with CRC tumor progression including lossof miR-133a and gain of miR-224. We identified 11 common miRNAs that weredifferentially expressed between normal colon and CRC in both the cell linemodels and clinical samples. In vitro functional studies indicated that miR-143and miR-145 appear to function in opposing manners to either inhibit or augmentcell proliferation in a metastatic CRC model. The pathways targeted by miR-143and miR-145 showed no significant overlap. Furthermore, gene expression analysis of metastatic versus non-metastatic isogenic cell lines indicated that miR-145targets involved in cell cycle and neuregulin pathways were significantlydown-regulated in the metastatic context.CONCLUSION: MiRNAs showing altered expression at different stages of CRC could betargets for CRC therapies and be further developed as potential diagnostic andprognostic analytes. The identified biological processes and signalling pathways collectively targeted by co-expressed miRNAs in CRC provide a basis forunderstanding the functional role of miRNAs in cancer. PMID: 19843336 |
| Expressiion Profile | Description: Characterization of microRNA expression in colorectal cancer Organism: Homo sapiens GEO ID: GSE10259 Platform: GPL4411 Number of samples: 66 |
| Design and Sample | Cancer Type: colorectal cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: colorectal cancer Control Sample: normal colon Num of Case: 59 Num of Control: 7 Quantification Software: Limma Num of miRNAs: 281 |
| Identification | Num of Up: 10 Num of Down: 19 |