| Experiment ID | EXP00054 |
| Reference | Title: The E2F3-Oncomir-1 axis is activated in Wilms' tumor. Author: Kort EJ, Farber L, Tretiakova M, Petillo D, Furge KA, Yang XJ, Cornelius A,Teh BT. Journal: Cancer Res. 2008 Jun 1;68(11):4034-8. doi: 10.1158/0008-5472.CAN-08-0592. Abstract: Oncomir-1 is an oncogenic cluster of microRNAs (miRNA) located on chromosome 13. Previous in vitro studies showed that it is transcriptionally regulated by thetranscription factor E2F3. In this report, we combine expression profiling ofboth mRNA and miRNAs in Wilms' tumor (WT) samples to provide the first evidencethat the E2F3-Oncomir-1 axis, previously identified in cell culture, isderegulated in primary human tumors. Analysis of RNA expression signatures showedthat an E2F3 gene signature was activated in all WT samples analyzed, in contrastto other kidney tumors. This finding was validated by immunohistochemistry on theprotein level. Expression of E2F3 was lowest in early-stage tumors and highest inmetastatic tissue. Expression profiling of miRNAs in WT showed that expression ofeach measured member of the Oncomir-1 family was highest in WT relative to other kidney tumor subtypes. Quantitative PCR confirmed that these miRNAs wereoverexpressed in WT relative to normal kidney tissue. These results suggest that the E2F3-Oncomir-1 axis is activated in WT. Our study also shows the utility ofintegrated genomics combining gene signature analysis with miRNA expressionprofiling to identify protein-miRNA interactions that are perturbed in diseasestates. PMID: 18519660 |
| Expressiion Profile | Description: miRNA profiling of adult and pediatric renal tumors Organism: Homo sapiens GEO ID: GSE11016 Platform: GPL6668 Number of samples: 32 |
| Design and Sample | Cancer Type: kidney cancer Cancer SubType: Wilms tumor Cell Line: N/D Experimental Design: cancer vs normal Case Sample: Wilms tumor Control Sample: normal kidney Num of Case: 12 Num of Control: 12 Quantification Software: Limma Num of miRNAs: 148 |
| Identification | Num of Up: 47 Num of Down: 56 |