| Experiment ID | EXP00061 |
| Reference | Title: MicroRNA expression ratio is predictive of head and neck squamous cell carcinoma. Author: Avissar M, Christensen BC, Kelsey KT, Marsit CJ. Journal: Clin Cancer Res. 2009 Apr 15;15(8):2850-5. doi: 10.1158/1078-0432.CCR-08-3131.Epub 2009 Apr 7. Abstract: PURPOSE: The involvement of microRNAs in cancer and their potential as biomarkersof diagnosis and prognosis are becoming increasingly appreciated. We sought toidentify microRNAs altered in head and neck squamous cell carcinoma (HNSCC) andto determine whether microRNA expression is predictive of disease.EXPERIMENTAL DESIGN: RNA isolated from fresh-frozen primary tumors, fresh-frozen nondiseased head and neck epithelial tissues, and HNSCC cell lines was profiledfor the expression of 662 microRNAs by microarray. The microRNAs that were bothdifferentially expressed on the array and by quantitative reversetranscription-PCR were subsequently validated by quantitative reversetranscription-PCR using a total of 99 HNSCC samples and 14 normal epithelia.RESULTS: A marked difference in microRNA expression pattern was observed between tumors and cell lines. Eighteen microRNAs were significantly altered in theirexpression between normal tissues and tumors. Four of these microRNAs werevalidated in the larger sample series, and each showed significant differentialexpression (P < 0.0001). Furthermore, an expression ratio of miR-221:miR-375showed a high sensitivity (0.92) and specificity (0.93) for disease prediction.CONCLUSIONS: These data suggest that cultured tumor cell lines are inappropriate for microRNA biomarker identification and that the pattern of microRNA expressionin primary head and neck tissues is reflective of disease status, with certainmicroRNAs exhibiting strong predictive potential. These results indicate thatmiR-221 and miR-375 should be evaluated further as diagnostic biomarkers because they may hold utility in defining broadly responsive prevention and treatmentstrategies for HNSCC. PMID: 19351747 |
| Expressiion Profile | Description: miRNA expression in head and neck squamous cell carcinoma (HNSCC) Organism: Homo sapiens GEO ID: GSE11163 Platform: GPL6690 Number of samples: 27 |
| Design and Sample | Cancer Type: head and neck cancer Cancer SubType: HPV negative Cell Line: N/D Experimental Design: cancer vs normal Case Sample: head and neck cancer HPV negative Control Sample: normal head and neck epithelium Num of Case: 13 Num of Control: 5 Quantification Software: Limma Num of miRNAs: 328 |
| Identification | Num of Up: 11 Num of Down: 7 |