| Experiment ID | EXP00064 |
| Reference | Title: Differentiation stage-specific expression of microRNAs in B lymphocytes anddiffuse large B-cell lymphomas. Author: Malumbres R, Sarosiek KA, Cubedo E, Ruiz JW, Jiang X, Gascoyne RD, Tibshirani R, Lossos IS. Journal: Blood. 2009 Apr 16;113(16):3754-64. doi: 10.1182/blood-2008-10-184077. Epub 2008 Dec 1. Abstract: miRNAs are small RNA molecules binding to partially complementary sites in the3'-UTR of target transcripts and repressing their expression. miRNAs orchestrate multiple cellular functions and play critical roles in cell differentiation andcancer development. We analyzed miRNA profiles in B-cell subsets duringperipheral B-cell differentiation as well as in diffuse large B-cell lymphoma(DLBCL) cells. Our results show temporal changes in the miRNA expression duringB-cell differentiation with a highly unique miRNA profile in germinal center (GC)lymphocytes. We provide experimental evidence that these changes may bephysiologically relevant by demonstrating that GC-enriched hsa-miR-125bdown-regulates the expression of IRF4 and PRDM1/BLIMP1, and memory Bcell-enriched hsa-miR-223 down-regulates the expression of LMO2. We furtherdemonstrate that although an important component of the biology of a malignantcell is inherited from its nontransformed cellular progenitor-GCcentroblasts-aberrant miRNA expression is acquired upon cell transformation. A9-miRNA signature was identified that could precisely differentiate the 2 majorsubtypes of DLBCL. Finally, expression of some of the miRNAs in this signature iscorrelated with clinical outcome of uniformly treated DLBCL patients. PMID: 19047678 |
| Expressiion Profile | Description: miRNA profiles of diffuse large B cell lymphoma (DLBCL) derived cell lines Organism: Homo sapiens GEO ID: GSE12933 Platform: GPL7373 Number of samples: 8 |
| Design and Sample | Cancer Type: lymphoma Cancer SubType: diffuse large B cell lymphoma Cell Line: N/D Experimental Design: subtype1 vs substype2 Case Sample: Activated B Cell like (ABC-DLBCL) Control Sample: Germinal Center B cell like (GCB-DLBCL) Num of Case: 5 Num of Control: 3 Quantification Software: Limma Num of miRNAs: 170 |
| Identification | Num of Up: 1 Num of Down: 0 |