| Experiment ID | EXP00069 |
| Reference | Title: Diagnostic and prognostic implications of microRNA profiling in prostatecarcinoma. Author: Schaefer A, Jung M, Mollenkopf HJ, Wagner I, Stephan C, Jentzmik F, Miller K, Lein M, Kristiansen G, Jung K. Journal: Int J Cancer. 2010 Mar 1;126(5):1166-76. doi: 10.1002/ijc.24827. Abstract: This study aimed to investigate the microRNA (miRNA) profile in prostatecarcinoma tissue by microarray analysis and RT-qPCR, to clarify associations ofmiRNA expression with clinicopathologic data and to evaluate the potential ofmiRNAs as diagnostic and prognostic markers. Matched tumor and adjacent normaltissues were obtained from 76 radical prostatectomy specimens. Twenty-four tissuepairs were analyzed using human miRNA microarrays for 470 human miRNAs.Differentially expressed miRNAs were validated by TaqMan RT-qPCR using all 76tissue pairs. The diagnostic potential of miRNAs was calculated by receiveroperating characteristics analyses. The prognostic value was assessed in terms ofbiochemical recurrence using Kaplan-Meier and Cox regression analyses. Fifteendifferentially expressed miRNAs were identified with concordant fold-changes bymicroarray and RT-qPCR analyses. Ten microRNAs (hsa-miR-16, hsa-miR-31,hsa-miR-125b, hsa-miR-145, hsa-miR-149, hsa-miR-181b, hsa-miR-184, hsa-miR-205,hsa-miR-221, hsa-miR-222) were downregulated and 5 miRNAs (hsa-miR-96,hsa-miR-182, hsa-miR-182, hsa-miR-183, hsa-375) were upregulated. Expression of 5miRNAs correlated with Gleason score or pathological tumor stage. Already 2microRNAs classified up to 84% of malignant and nonmalignant samples correctly.Expression of hsa-miR-96 was associated with cancer recurrence after radicalprostatectomy and that prognostic information was confirmed by an independenttumor sample set from 79 patients. That was shown with hsa-miR-96 and the Gleasonscore as final variables in the Cox models build in the 2 patient setsinvestigated. Thus, differential miRNAs in prostate cancer are useful diagnostic and prognostic indicators. This study provides a solid basis for furtherfunctional analyses of miRNAs in prostate cancer. PMID: 19676045 |
| Expressiion Profile | Description: MicroRNA Profiling Identifies Potential Diagnostic and Prognostic Markers in Prostate Cancer Organism: Homo sapiens GEO ID: GSE14857 Platform: GPL6955 Number of samples: 24 |
| Design and Sample | Cancer Type: prostate cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: prostate cancer Control Sample: normal prostate Num of Case: 12 Num of Control: 12 Quantification Software: Limma Num of miRNAs: 470 |
| Identification | Num of Up: 3 Num of Down: 2 |