| Experiment ID | EXP00075 |
| Reference | Title: MicroRNA expression signatures in Barrett's esophagus and esophagealadenocarcinoma. Author: Yang H, Gu J, Wang KK, Zhang W, Xing J, Chen Z, Ajani JA, Wu X. Journal: Clin Cancer Res. 2009 Sep 15;15(18):5744-52. doi: 10.1158/1078-0432.CCR-09-0385. Epub 2009 Sep 8. Abstract: PURPOSE: Esophageal adenocarcinoma is a highly aggressive malignancy thatfrequently develops from Barrett's esophagus, a premalignant pathologic changeoccurring in the lower end of the esophagus. Identifying Barrett's esophaguspatients at high risk of malignant transformation is essential to the prevention of esophageal adenocarcinoma. Although microRNA (miRNA) expression signatureshave been associated with the etiology and prognosis of several types of cancers,their roles in the development of esophageal adenocarcinoma have not beenextensively evaluated.EXPERIMENTAL DESIGN: In this study, we analyzed the expression patterns of 470human miRNAs using Agilent miRNA microarray in 32 disease/normal-paired tissuesfrom 16 patients diagnosed with Barrett's esophagus of either low- or high-grade dysplasia, or esophageal adenocarcinoma.RESULTS: Using unsupervised hierarchical clustering and class comparisonanalyses, we found that miRNA expression profiles in tissues of Barrett'sesophagus with high-grade dysplasia were significantly different from theircorresponding normal tissues. Similar findings were observed for esophagealadenocarcinoma, but not for Barrett's esophagus with low-grade dysplasia. Theexpression patterns of selected miRNAs were further validated using quantitative reverse transcription real-time PCR in an independent set of 75 pairs ofdisease/normal tissues. Finally, we identified several miRNAs that were involved in the progressions from low grade-dysplasia Barrett's esophagus to esophagealadenocarcinoma.CONCLUSIONS: We showed that miRNAs were involved in the development andprogression of esophageal adenocarcinoma. The identified significant miRNAs that may become potential targets for early detection, chemoprevention, and treatment of esophageal cancer. PMID: 19737949 |
| Expressiion Profile | Description: MicroRNA expression signatures in Barrett's esophagus and esophageal adenocarcinoma Organism: Homo sapiens GEO ID: GSE16456 Platform: GPL6955 Number of samples: 32 |
| Design and Sample | Cancer Type: esophageal cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: esophageal cancer Control Sample: normal esophagus Num of Case: 6 Num of Control: 6 Quantification Software: Limma Num of miRNAs: 470 |
| Identification | Num of Up: 46 Num of Down: 54 |