| Experiment ID | EXP00077 |
| Reference | Title: Downregulated microRNA-200a in meningiomas promotes tumor growth by reducingE-cadherin and activating the Wnt/beta-catenin signaling pathway. Author: Saydam O, Shen Y, Würdinger T, Senol O, Boke E, James MF, Tannous BA,Stemmer-Rachamimov AO, Yi M, Stephens RM, Fraefel C, Gusella JF, Krichevsky AM,Breakefield XO. Journal: Mol Cell Biol. 2009 Nov;29(21):5923-40. doi: 10.1128/MCB.00332-09. Epub 2009 Aug 24. Abstract: Meningiomas, one of the most common human brain tumors, are derived fromarachnoidal cells associated with brain meninges, are usually benign, and arefrequently associated with neurofibromatosis type 2. Here, we define a typicalhuman meningioma microRNA (miRNA) profile and characterize the effects of onedownregulated miRNA, miR-200a, on tumor growth. Elevated levels of miR-200ainhibited meningioma cell growth in culture and in a tumor model in vivo.Upregulation of miR-200a decreased the expression of transcription factors ZEB1and SIP1, with consequent increased expression of E-cadherin, an adhesion proteinassociated with cell differentiation. Downregulation of miR-200a in meningiomasand arachnoidal cells resulted in increased expression of beta-catenin and cyclinD1 involved in cell proliferation. miR-200a was found to directly targetbeta-catenin mRNA, thereby inhibiting its translation and blockingWnt/beta-catenin signaling, which is frequently involved in cancer. A directcorrelation was found between the downregulation of miR-200a and the upregulationof beta-catenin in human meningioma samples. Thus, miR-200a appears to act as amultifunctional tumor suppressor miRNA in meningiomas through effects on theE-cadherin and Wnt/beta-catenin signaling pathways. This reveals a previouslyunrecognized signaling cascade involved in meningioma tumor development andhighlights a novel molecular interaction between miR-200a and Wnt signaling,thereby providing insights into novel therapies for meningiomas. PMID: 19703993 |
| Expressiion Profile | Description: microRNA profiles of meningiomas Organism: Homo sapiens GEO ID: GSE17792 Platform: GPL7436 Number of samples: 17 |
| Design and Sample | Cancer Type: brain cancer Cancer SubType: meningioma Cell Line: N/D Experimental Design: cancer vs normal Case Sample: meningioma tumor sample Control Sample: arachnoidal tissue Num of Case: 14 Num of Control: 3 Quantification Software: Limma Num of miRNAs: 376 |
| Identification | Num of Up: 8 Num of Down: 0 |