| Experiment ID | EXP00079 |
| Reference | Title: MicroRNA expression profiling in human Barrett's carcinogenesis. Author: Fassan M, Volinia S, Palatini J, Pizzi M, Baffa R, De Bernard M, Battaglia G, Parente P, Croce CM, Zaninotto G, Ancona E, Rugge M. Journal: Int J Cancer. 2011 Oct 1;129(7):1661-70. doi: 10.1002/ijc.25823. Epub 2011 Mar11. Abstract: Barrett's esophagus (BE) is characterized by the native stratified squamousepithelium (N) lining the esophagus being replaced by a columnar epithelium with intestinal differentiation (Barrett's mucosa; BM). BM is considered as the mainrisk factor for esophageal adenocarcinoma (Barrett's adenocarcinoma; BAc).MicroRNAs (miRNAs) are a class of small noncoding RNAs that control geneexpression by targeting messenger RNAs and they are reportedly dysregulated inBM. To test the hypothesis that a specific miRNA expression signaturecharacterizes BM development and progression, we performed miRNA microarrayanalysis comparing native esophageal mucosa with all the phenotypic lesions seen in the Barrett's carcinogenic process. Specimens were collected from 14 BEpatients who had undergone esophagectomy, including: 14 with N, 14 with BM, 7with low-grade intraepithelial neoplasia, 5 with high-grade intra-epithelialneoplasia and 11 with BAc. Microarray findings were further validated byquantitive real-time polymerase chain reaction and in situ hybridization analysesusing a different series of consecutive cases (162 biopsy samples and 5esophagectomies) of histologically proven, long-segment BE. We identified a miRNAsignature of Barrett's carcinogenesis consisting of an increased expression of 6 miRNAs and a reduced expression of 7 miRNAs. To further support these results, weinvestigated target gene expression using the Oncomine database and/orimmunohistochemical analysis. We found that target gene expression correlatedsignificantly with miRNA dysregulation. Specific miRNAs are directly involved in BE progression to cancer. miRNA profiling significantly expands current knowledgeon the molecular history of Barrett's carcinogenesis, also identifying molecular markers of cancer progression. PMID: 21128279 |
| Expressiion Profile | Description: microRNA/UCR expression profiling of human Barrett's carcinogenesis Organism: Homo sapiens GEO ID: GSE20099 Platform: GPL8871 Number of samples: 51 |
| Design and Sample | Cancer Type: esophageal cancer Cancer SubType: Barrett's carcinogenesis Cell Line: N/D Experimental Design: cancer vs normal Case Sample: low-grade intra-epithelial neoplasia Control Sample: normal esophagus Num of Case: 5 Num of Control: 14 Quantification Software: Limma Num of miRNAs: 605 |
| Identification | Num of Up: 33 Num of Down: 25 |