| Experiment ID | EXP00083 |
| Reference | Title: Expression of microRNAs and protein-coding genes associated with perineuralinvasion in prostate cancer. Author: Prueitt RL, Yi M, Hudson RS, Wallace TA, Howe TM, Yfantis HG, Lee DH, StephensRM, Liu CG, Calin GA, Croce CM, Ambs S. Journal: Prostate. 2008 Aug 1;68(11):1152-64. doi: 10.1002/pros.20786. Abstract: BACKGROUND: Perineural invasion (PNI) is the dominant pathway for local invasion in prostate cancer. To date, only few studies have investigated the moleculardifferences between prostate tumors with PNI and those without it.METHODS: To evaluate the involvement of both microRNAs and protein-coding genesin PNI, we determined their genome-wide expression with a custom microRNAmicroarray and Affymetrix GeneChips in 50 prostate adenocarcinomas with PNI and 7without it. In situ hybridization (ISH) and immunohistochemistry was used tovalidate candidate genes.RESULTS: Unsupervised classification of the 57 adenocarcinomas revealed twoclusters of tumors with distinct global microRNA expression. One clustercontained all non-PNI tumors and a subgroup of PNI tumors. Significance analysis of microarray data yielded a list of microRNAs associated with PNI. At a falsediscovery rate (FDR)<10%, 19 microRNAs were higher expressed in PNI tumors thanin non-PNI tumors. The most differently expressed microRNA was miR-224. ISHshowed that this microRNA is expressed by perineural cancer cells. The analysisof protein-coding genes identified 34 transcripts that were differently expressedby PNI status (FDR<10%). These transcripts were down-regulated in PNI tumors.Many of those encoded metallothioneins and proteins with mitochondriallocalization and involvement in cell metabolism. Consistent with the microarraydata, perineural cancer cells tended to have lower metallothionein expression by immunohistochemistry than nonperineural cancer cells.CONCLUSIONS: Although preliminary, our findings suggest that alterations inmicroRNA expression, mitochondrial function, and cell metabolism occur at thetransition from a noninvasive prostate tumor to a tumor with PNI. PMID: 18459106 |
| Expressiion Profile | Description: Expression of microRNAs and Protein-coding Genes Associated with Perineural Invasion in Prostate Cancer Organism: Homo sapiens GEO ID: GSE7055 Platform: GPL4700 Number of samples: 114 |
| Design and Sample | Cancer Type: prostate cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: high grade vs low grade Case Sample: prostate cancer gleason sum 7 Control Sample: prostate adenocarcinoma gleason sum 6 Num of Case: 41 Num of Control: 13 Quantification Software: Limma Num of miRNAs: 230 |
| Identification | Num of Up: 3 Num of Down: 0 |