| Experiment ID | EXP00084 |
| Reference | Title: MicroRNA expression profiles associated with prognosis and therapeutic outcome incolon adenocarcinoma. Author: Schetter AJ, Leung SY, Sohn JJ, Zanetti KA, Bowman ED, Yanaihara N, Yuen ST,Chan TL, Kwong DL, Au GK, Liu CG, Calin GA, Croce CM, Harris CC. Journal: JAMA. 2008 Jan 30;299(4):425-36. doi: 10.1001/jama.299.4.425. Abstract: CONTEXT: MicroRNAs have potential as diagnostic biomarkers and therapeutictargets in cancer. No study has evaluated the association between microRNAexpression patterns and colon cancer prognosis or therapeutic outcome.OBJECTIVE: To identify microRNA expression patterns associated with colonadenocarcinomas, prognosis, or therapeutic outcome.DESIGN, SETTING, AND PATIENTS: MicroRNA microarray expression profiling of tumorsand paired nontumorous tissues was performed on a US test cohort of 84 patientswith incident colon adenocarcinoma, recruited between 1993 and 2002. We evaluatedassociations with tumor status, TNM staging, survival prognosis, and response to adjuvant chemotherapy. Associations were validated in a second, independentChinese cohort of 113 patients recruited between 1991 and 2000, usingquantitative reverse transcription polymerase chain reaction assays. The finaldate of follow-up was December 31, 2005, for the Maryland cohort and August 16,2004, for the Hong Kong cohort.MAIN OUTCOME MEASURES: MicroRNAs that were differentially expressed in tumors andmicroRNA expression patterns associated with survival using cancer-specific deathas the end point. RESULTS Thirty-seven microRNAs were differentially expressed intumors from the test cohort. Selected for validation were miR-20a, miR-21,miR-106a, miR-181b, and miR-203, and all 5 were enriched in tumors from thevalidation cohort (P < .001). Higher miR-21 expression was present in adenomas (P= .006) and in tumors with more advanced TNM staging (P < .001). In situhybridization demonstrated miR-21 to be expressed at high levels in coloniccarcinoma cells. The 5-year cancer-specific survival rate was 57.5% for theMaryland cohort and was 49.5% for the Hong Kong cohort. High miR-21 expressionwas associated with poor survival in both the training (hazard ratio, 2.5; 95%confidence interval, 1.2-5.2) and validation cohorts (hazard ratio, 2.4; 95%confidence interval, 1.4-3.9), independent of clinical covariates, including TNM staging, and was associated with a poor therapeutic outcome.CONCLUSIONS: Expression patterns of microRNAs are systematically altered in colonadenocarcinomas. High miR-21 expression is associated with poor survival and poortherapeutic outcome. PMID: 18230780 |
| Expressiion Profile | Description: MicroRNA profiles of 84 colon adenocarcinomas and paired nontumorous Organism: Homo sapiens GEO ID: GSE7828 Platform: GPL4700 Number of samples: 170 |
| Design and Sample | Cancer Type: colon cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: colon tumour Control Sample: normal colon Num of Case: 85 Num of Control: 85 Quantification Software: Limma Num of miRNAs: 230 |
| Identification | Num of Up: 19 Num of Down: 8 |