| Experiment ID | EXP00097 |
| Reference | Title: Regulation of insulin-like growth factor-mammalian target of rapamycin signaling by microRNA in childhood adrenocortical tumors. Author: Doghman M, El Wakil A, Cardinaud B, Thomas E, Wang J, Zhao W, Peralta-DelValle MH, Figueiredo BC, Zambetti GP, Lalli E. Journal: Cancer Res. 2010 Jun 1;70(11):4666-75. doi: 10.1158/0008-5472.CAN-09-3970. Epub2010 May 18. Abstract: MicroRNAs (miRNAs) act at the posttranscriptional level to control geneexpression in virtually every biological process, including oncogenesis. Here, wereport the identification of a set of miRNAs that are differentially regulated inchildhood adrenocortical tumors (ACT), including miR-99a and miR-100. Functional analysis of these miRNAs in ACT cell lines showed that they coordinately regulateexpression of the insulin-like growth factor-mammalian target of rapamycin(mTOR)-raptor signaling pathway through binding sites in their 3'-untranslatedregions. In these cells, the active Ser(2448)-phosphorylated form of mTOR ispresent only in mitotic cells in association with the mitotic spindle and midbodyin the G(2)-M phases of the cell cycle. Pharmacologic inhibition of mTORsignaling by everolimus greatly reduces tumor cell growth in vitro and in vivo.Our results reveal a novel mechanism of regulation of mTOR signaling by miRNAs,and they lay the groundwork for clinical evaluation of drugs inhibiting the mTOR pathway for treatment of adrenocortical cancer. PMID: 20484036 |
| Expressiion Profile | Description: Study of miRNA expression in childhood adrenocortical tumors Organism: Homo sapiens GEO ID: GSE19856 Platform: GPL8227 Number of samples: 30 |
| Design and Sample | Cancer Type: adrenocortical cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: adrenocortical tumor Control Sample: normal Num of Case: 25 Num of Control: 5 Quantification Software: Limma Num of miRNAs: 723 |
| Identification | Num of Up: 0 Num of Down: 1 |