| Experiment ID | EXP00098 |
| Reference | Title: MicroRNA expression profiles associated with mutational status and survival inmalignant melanoma. Author: Caramuta S, Egyházi S, Rodolfo M, Witten D, Hansson J, Larsson C, Lui WO. Journal: J Invest Dermatol. 2010 Aug;130(8):2062-70. doi: 10.1038/jid.2010.63. Epub 2010Apr 1. Abstract: Malignant cutaneous melanoma is a highly aggressive form of skin cancer. Despite improvements in early melanoma diagnosis, the 5-year survival rate remains low inadvanced disease. Therefore, novel biomarkers are urgently needed to devise newmeans of detection and treatment. In this study, we aimed to improve ourunderstanding of microRNA (miRNA) deregulation in melanoma development and their impact on patient survival. Global miRNA expression profiles of a set of melanomalymph node metastases, melanoma cell lines, and melanocyte cultures weredetermined using Agilent array. Deregulated miRNAs were evaluated in relationwith clinical characteristics, patient survival, and mutational status for BRAFand NRAS. Several miRNAs were differentially expressed between melanocytes andmelanomas as well as melanoma cell lines. In melanomas, miR-193a, miR-338, andmiR-565 were underexpressed in cases with a BRAF mutation. Furthermore, lowexpression of miR-191 and high expression of miR-193b were associated with poormelanoma-specific survival. In conclusion, our findings show miRNA dysregulation in malignant melanoma and its relation to established molecular backgrounds ofBRAF and NRAS oncogenic mutations. The identification of an miRNA classifier for poor survival may lead to the development of miRNA detection as a complementaryprognostic tool in clinical practice. PMID: 20357817 |
| Expressiion Profile | Description: MicroRNA Expression Profiles Associated with Mutational Status and Survival in Malignant Melanoma Organism: Homo sapiens GEO ID: GSE19387 Platform: GPL9081 Number of samples: 40 |
| Design and Sample | Cancer Type: melanoma Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: melanoma cell line Control Sample: normal melanocyte Num of Case: 21 Num of Control: 3 Quantification Software: Limma Num of miRNAs: 167 |
| Identification | Num of Up: 10 Num of Down: 9 |