| Experiment ID | EXP00100 |
| Reference | Title: microRNA-122 as a regulator of mitochondrial metabolic gene network inhepatocellular carcinoma. Author: Burchard J, Zhang C, Liu AM, Poon RT, Lee NP, Wong KF, Sham PC, Lam BY,Ferguson MD, Tokiwa G, Smith R, Leeson B, Beard R, Lamb JR, Lim L, Mao M, Dai H, Luk JM. Journal: Mol Syst Biol. 2010 Aug 24;6:402. doi: 10.1038/msb.2010.58. Abstract: Tumorigenesis involves multistep genetic alterations. To elucidate the microRNA(miRNA)-gene interaction network in carcinogenesis, we examined their genome-wideexpression profiles in 96 pairs of tumor/non-tumor tissues from hepatocellularcarcinoma (HCC). Comprehensive analysis of the coordinate expression of miRNAsand mRNAs reveals that miR-122 is under-expressed in HCC and that increasedexpression of miR-122 seed-matched genes leads to a loss of mitochondrialmetabolic function. Furthermore, the miR-122 secondary targets, which decrease inexpression, are good prognostic markers for HCC. Transcriptome profiling datafrom additional 180 HCC and 40 liver cirrhotic patients in the same cohort wereused to confirm the anti-correlation of miR-122 primary and secondary target genesets. The HCC findings can be recapitulated in mouse liver by silencing miR-122with antagomir treatment followed by gene-expression microarray analysis. Invitro miR-122 data further provided a direct link between induction ofmiR-122-controlled genes and impairment of mitochondrial metabolism. Inconclusion, miR-122 regulates mitochondrial metabolism and its loss may bedetrimental to sustaining critical liver function and contribute to morbidity andmortality of liver cancer patients. PMID: 20739924 |
| Expressiion Profile | Description: miR-122 as a Regulator of Mitochondrial Metabolic Gene Network in Hepatocellular Carcinoma Organism: Homo sapiens GEO ID: GSE22058 Platform: GPL10457 Number of samples: 397 |
| Design and Sample | Cancer Type: hepatocellular carcinoma Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: hepatocellular carcinoma Control Sample: normal liver Num of Case: 96 Num of Control: 96 Quantification Software: Limma Num of miRNAs: 220 |
| Identification | Num of Up: 83 Num of Down: 63 |