| Experiment ID | EXP00108 |
| Reference | Title: MicroRNA profiles of prostate carcinoma detected by multiplatform microRNAscreening. Author: Wach S, Nolte E, Szczyrba J, Stöhr R, Hartmann A, Ørntoft T, Dyrskjøt L, EltzeE, Wieland W, Keck B, Ekici AB, Grässer F, Wullich B. Journal: Int J Cancer. 2012 Feb 1;130(3):611-21. doi: 10.1002/ijc.26064. Epub 2011 May 9. Abstract: MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression viaposttranscriptional inhibition of protein synthesis. They play a vital role intumorigenesis. To characterize the diagnostic potential of miRNAs in prostatecancer, a leading cause of cancer mortality, we performed screening of miRNAexpression profiles. We used commercially available microarrays to establishmiRNA expression profiles from a cohort of 20 cancer samples. The expression ofselected miRNAs was analyzed by quantitative real-time PCR and the identity ofmiRNA expressing cells was determined by miRNA in situ hybridization. Weidentified 25 miRNAs that showed a significant differential expression in cancer samples. The comparison with previously published data generated by deepsequencing of cDNA libraries of small RNA molecules revealed a concordance rateof 47% among miRNAs identified with both techniques. The differential expression of miRNAs miR-375, miR-143 and miR-145 was validated by quantitative PCR. MiRNAin situ hybridization revealed that the differential expression is cancer-cellassociated. A combination of three miRNAs correctly classified tissue sampleswith an accuracy of 77.6% with an area under the receiver-operator characteristiccurve of 0.810. Our data extend the knowledge about the deregulation of miRNAs inprostate cancer. The differential expression of several miRNAs is highlyconsistent using independent cohorts of tumor samples, different tissuepreservation methods and different experimental methods. Our results indicatethat combinations of miRNAs are promising biomarkers for the diagnosis ofprostate cancer. PMID: 21400514 |
| Expressiion Profile | Description: MicroRNA expression data from human prostate cancer Organism: Homo sapiens GEO ID: GSE23022 Platform: GPL8786 Number of samples: 40 |
| Design and Sample | Cancer Type: prostate cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: prostate cancer Control Sample: normal prostate Num of Case: 20 Num of Control: 20 Quantification Software: Limma Num of miRNAs: 597 |
| Identification | Num of Up: 10 Num of Down: 3 |