| Experiment ID | EXP00113 |
| Reference | Title: miR-193b Regulates Mcl-1 in Melanoma. Author: Chen J, Zhang X, Lentz C, Abi-Daoud M, Paré GC, Yang X, Feilotter HE, Tron VA. Journal: Am J Pathol. 2011 Nov;179(5):2162-8. doi: 10.1016/j.ajpath.2011.07.010. Epub 2011Sep 3. Abstract: MicroRNAs play important roles in gene regulation, and their expression isfrequently dysregulated in cancer cells. In a previous study, we reported thatmiR-193b represses cell proliferation and regulates cyclin D1 in melanoma cells, suggesting that miR-193b could act as a tumor suppressor. Herein, we demonstrate that miR-193b also down-regulates myeloid cell leukemia sequence 1 (Mcl-1) inmelanoma cells. MicroRNA microarray profiling revealed that miR-193b is expressedat a significantly lower level in malignant melanoma than in benign nevi.Consistent with this, Mcl-1 is detected at a higher level in malignant melanomathan in benign nevi. In a survey of melanoma samples, the level of Mcl-1 isinversely correlated with the level of miR-193b. Overexpression of miR-193b inmelanoma cells represses Mcl-1 expression. Previous studies showed that Mcl-1knockdown cells are hypersensitive to ABT-737, a small-molecule inhibitor ofBcl-2, Bcl-X(L), and Bcl-w. Similarly, overexpression of miR-193b restoresABT-737 sensitivity to ABT-737-resistant cells. Furthermore, the effect ofmiR-193b on the expression of Mcl-1 seems to be mediated by direct interactionbetween miR-193b and seed and seedless pairing sequences in the 3' untranslatedregion of Mcl-1 mRNA. Thus, this study provides evidence that miR-193b directlyregulates Mcl-1 and that down-regulation of miR-193b in vivo could be an earlyevent in melanoma progression. PMID: 21893020 |
| Expressiion Profile | Description: miR-193b Regulates Mcl-1 in Melanoma Organism: Homo sapiens GEO ID: GSE24996 Platform: GPL6955 Number of samples: 31 |
| Design and Sample | Cancer Type: melanoma Cancer SubType: N/D Cell Line: N/D Experimental Design: metastasis Case Sample: metastatic melanoma Control Sample: primary melanoma Num of Case: 8 Num of Control: 15 Quantification Software: Limma Num of miRNAs: 470 |
| Identification | Num of Up: 54 Num of Down: 71 |