| Experiment ID | EXP00118 |
| Reference | Title: Integrative network analysis reveals active microRNAs and their functions ingastric cancer. Author: Tseng CW, Lin CC, Chen CN, Huang HC, Juan HF. Journal: BMC Syst Biol. 2011 Jun 26;5:99. doi: 10.1186/1752-0509-5-99. Abstract: BACKGROUND: MicroRNAs (miRNAs) are a class of endogenous, small and highlyconserved noncoding RNAs that control gene expression either by degradation oftarget mRNAs or by inhibition of protein translation. They play important rolesin cancer progression. A single miRNA can provoke a chain reaction and furtheraffect protein interaction network (PIN). Therefore, we developed a novelintegrative approach to identify the functional roles and the regulated PIN ofoncomirs.RESULTS: We integrated the expression profiles of miRNA and mRNA with the humanPIN to reveal miRNA-regulated PIN in specific biological conditions. Thepotential functions of miRNAs were determined by functional enrichment analysisand the activities of miRNA-regulated PINs were evaluated by the co-expression ofprotein-protein interactions (PPIs). The function of a specific miRNA, miR-148a, was further examined by clinical data analysis and cell-based experiments. Weuncovered several miRNA-regulated networks which were enriched with functionsrelated to cancer progression. One miRNA, miR-148a, was identified and itsfunction is to decrease tumor proliferation and metastasis through its regulated PIN. Furthermore, we found that miR-148a could reduce the invasiveness, migratoryand adhesive activities of gastric tumor cells. Most importantly, elevatedmiR-148a level in gastric cancer tissues was strongly correlated with distantmetastasis, organ and peritoneal invasion and reduced survival rate.CONCLUSIONS: This study provides a novel method to identify active oncomirs andtheir potential functions in gastric cancer progression. The present data suggestthat miR-148a could be a potential prognostic biomarker of gastric cancer andfunction as a tumor suppressor through repressing the activity of its regulatedPIN. PMID: 21703006 |
| Expressiion Profile | Description: Expression profiles of microRNAs in gastric cancer Organism: Homo sapiens GEO ID: GSE28700 Platform: GPL9081 Number of samples: 44 |
| Design and Sample | Cancer Type: gastric cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: gastric cancer Control Sample: normal tissue Num of Case: 22 Num of Control: 22 Quantification Software: Limma Num of miRNAs: 470 |
| Identification | Num of Up: 28 Num of Down: 23 |