| Experiment ID | EXP00121 |
| Reference | Title: The role of microRNA deregulation in the pathogenesis of adrenocorticalcarcinoma. Author: Özata DM, Caramuta S, Velázquez-Fernández D, Akçakaya P, Xie H, Höög A,Zedenius J, Bäckdahl M, Larsson C, Lui WO. Journal: Endocr Relat Cancer. 2011 Oct 27;18(6):643-55. doi: 10.1530/ERC-11-0082. Print2011 Oct. Abstract: Adrenocortical carcinoma (ACC) is an aggressive tumor showing frequent metastaticspread and poor survival. Although recent genome-wide studies of ACC havecontributed to our understanding of the disease, major challenges remain for bothdiagnostic and prognostic assessments. The aim of this study was to identifyspecific microRNAs (miRNAs) associated with malignancy and survival of ACCpatients. miRNA expression profiles were determined in a series of ACC, adenoma, and normal cortices using microarray. A subset of miRNAs showed distinctexpression patterns in the ACC compared with adrenal cortices and adenomas. Amongothers, miR-483-3p, miR-483-5p, miR-210, and miR-21 were found overexpressed,while miR-195, miR-497, and miR-1974 were underexpressed in ACC. Inhibition ofmiR-483-3p or miR-483-5p and overexpression of miR-195 or miR-497 reduced cellproliferation in human NCI-H295R ACC cells. In addition, downregulation ofmiR-483-3p, but not miR-483-5p, and increased expression of miR-195 or miR-497led to significant induction of cell death. Protein expression of p53 upregulatedmodulator of apoptosis (PUMA), a potential target of miR-483-3p, wassignificantly decreased in ACC, and inversely correlated with miR-483-3pexpression. In addition, high expression of miR-503, miR-1202, and miR-1275 were found significantly associated with shorter overall survival among patients with ACC (P values: 0.006, 0.005, and 0.042 respectively). In summary, we identifiedadditional miRNAs associated with ACC, elucidated the functional role of fourmiRNAs in the pathogenesis of ACC cells, demonstrated the potential involvementof the pro-apoptotic factor PUMA (a miR-483-3p target) in adrenocortical tumors, and found novel miRNAs associated with survival in ACC. PMID: 21859927 |
| Expressiion Profile | Description: Deregulated miRNAs in adrenocortical tumors Organism: Homo sapiens GEO ID: GSE22816 Platform: GPL13347 Number of samples: 52 |
| Design and Sample | Cancer Type: adrenocortical cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: adrenocortical adenoma Control Sample: normal adreno cortex Num of Case: 26 Num of Control: 4 Quantification Software: Limma Num of miRNAs: 211 |
| Identification | Num of Up: 0 Num of Down: 3 |