| Experiment ID | EXP00138 |
| Reference | Title: Melanoma cell invasiveness is regulated by miR-211 suppression of the BRN2transcription factor. Author: Boyle GM, Woods SL, Bonazzi VF, Stark MS, Hacker E, Aoude LG, Dutton-Regester K, Cook AL, Sturm RA, Hayward NK. Journal: Pigment Cell Melanoma Res. 2011 Jun;24(3):525-37. doi:10.1111/j.1755-148X.2011.00849.x. Epub 2011 Apr 7. Abstract: To identify microRNAs potentially involved in melanomagenesis, we comparedmicroRNA expression profiles between melanoma cell lines and culturedmelanocytes. The most differentially expressed microRNA between the normal andtumor cell lines was miR-211. We focused on this pigment-cell-enriched miRNA asit is derived from the microphthalmia-associated transcription factor(MITF)-regulated gene, TRPM1 (melastatin). We find that miR-211 expression isgreatly decreased in melanoma cells and melanoblasts compared to melanocytes.Bioinformatic analysis identified a large number of potential targets of miR-211,including POU3F2 (BRN2). Inhibition of miR-211 in normal melanocytes resulted in increased BRN2 protein, indicating that endogenous miR-211 represses BRN2 indifferentiated cells. Over-expression of miR-211 in melanoma cell lines changedthe invasive potential of the cells in vitro through directly targeting BRN2translation. We propose a model for the apparent non-overlapping expressionlevels of BRN2 and MITF in melanoma, mediated by miR-211 expression. PMID: 21435193 |
| Expressiion Profile | Description: miRNA profiling of Melanoma Cell lines and Normal Melanocytes Organism: Homo sapiens GEO ID: GSE25653 Platform: GPL6955 Number of samples: 56 |
| Design and Sample | Cancer Type: melanoma Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: melanoma Control Sample: Melanocyte Num of Case: 52 Num of Control: 4 Quantification Software: Limma Num of miRNAs: 470 |
| Identification | Num of Up: 13 Num of Down: 15 |