| Experiment ID | EXP00142 |
| Reference | Title: Colorectal cancers with microsatellite instability display unique miRNA profiles. Author: Balaguer F, Moreira L, Lozano JJ, Link A, Ramirez G, Shen Y, Cuatrecasas M,Arnold M, Meltzer SJ, Syngal S, Stoffel E, Jover R, Llor X, Castells A, BolandCR, Gironella M, Goel A. Journal: Clin Cancer Res. 2011 Oct 1;17(19):6239-49. doi: 10.1158/1078-0432.CCR-11-1424.Epub 2011 Aug 15. Abstract: PURPOSE: microRNAs (miRNA) are small noncoding transcripts that play an importantrole in carcinogenesis. miRNA expression profiles have been shown to discriminatebetween different types of cancers. The aim of this study was to analyze globalmiRNA signatures in various groups of colorectal cancers (CRC) based on thepresence of microsatellite instability (MSI).EXPERIMENTAL DESIGN: We analyzed genome-wide miRNA expression profiles in 54 CRC tissues [22 with Lynch syndrome, 13 with sporadic MSI due to MLH1 methylation, 19without MSI (or microsatellite stable, MSS)] and 20 normal colonic tissues bymiRNA microarrays. Using an independent set of MSI-positive samples (13 withLynch syndrome and 20 with sporadic MSI), we developed a miRNA-based predictor todifferentiate both types of MSI by quantitative reverse transcriptase PCR.RESULTS: We found that the expression of a subset of nine miRNAs significantlydiscriminated between tumor and normal colonic mucosa tissues (overall error rate= 0.04). More importantly, Lynch syndrome tumors displayed a unique miRNA profilecompared with sporadic MSI tumors; miR-622, miR-1238, and miR-192 were the mostdifferentially expressed miRNAs between these two groups. We developed amiRNA-based predictor capable of differentiating between types of MSI in anindependent sample set.CONCLUSIONS: CRC tissues show distinct miRNA expression profiles compared withnormal colonic mucosa. The discovery of unique miRNA expression profiles that cansuccessfully discriminate between Lynch syndrome, sporadic MSI, and sporadic MSS colorectal cancers provides novel insights into the role of miRNAs in colorectal carcinogenesis, which may contribute to the diagnosis, prognosis, and treatmentof this disease. PMID: 21844009 |
| Expressiion Profile | Description: Colorectal cancers with microsatellite instability display unique miRNA profiles Organism: Homo sapiens GEO ID: GSE30454 Platform: GPL8179 Number of samples: 74 |
| Design and Sample | Cancer Type: colorectal cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: Lynch syndrome tumor Control Sample: normal colon Num of Case: 13 Num of Control: 20 Quantification Software: Limma Num of miRNAs: 846 |
| Identification | Num of Up: 51 Num of Down: 77 |