| Experiment ID | EXP00156 |
| Reference | Title: microRNA-145 in Barrett's oesophagus: regulating BMP4 signalling via GATA6. Author: van Baal JW, Verbeek RE, Bus P, Fassan M, Souza RF, Rugge M, ten Kate FJ,Vleggaar FP, Siersema PD. Journal: Gut. 2013 May;62(5):664-75. doi: 10.1136/gutjnl-2011-301061. Epub 2012 Apr 13. Abstract: OBJECTIVE: Barrett's oesophagus (BE) is a metaplastic condition of the distaloesophagus which predisposes to oesophageal adenocarcinoma (EAC). It has beensuggested that microRNAs (miRNAs) are involved in the process of development ofBE and EAC; however, few functional miRNA data are available. The aim of thestudy was to perform a tissue-specific miRNA profile and, based on this, toexamine the function of miRNA-145 in the oesophagus.DESIGN: miRNA expression profiling using microarray analysis in EAC, BE andnormal squamous epithelium of the oesophagus (SQ) was performed and validatedusing real-time PCR in samples from 15 patients and in situ hybridisation insamples from 10 patients. The proliferative effect of miRNA-145 precursortransfection in the SQ (HET-1A) and BE cell line (BAR-T) was measured. Downstreamtargets of miRNA-145 were determined by analysing mRNA and protein expressionfrom miRNA-145 transfected cells.RESULTS: Three unique miRNA expression profiles were found in tissue from EAC, BEand SQ, which showed that miRNA-145 was upregulated in BE compared with EAC andSQ. Overexpression of miRNA-145 in HET-1A and BAR-T cells reduced cellproliferation and inhibited GATA6, BMP4 and SOX9 mRNA expression. Furthermore,altered BMP4 signalling was observed in vitro on miRNA-145 overexpression. These effects were blocked when cells were co-transfected with a miRNA-145 specificinhibitor. Additionally, BMP4 incubation of HET-1A cells altered miRNA-145 andGATA6 expression over time.CONCLUSION: These results imply that miRNA-145 indirectly targets BMP4 via GATA6 and is potentially involved in the development of BE. PMID: 22504665 |
| Expressiion Profile | Description: microRNA expression in Barrett's esophagus and esophageal adenocarcinoma Organism: Homo sapiens GEO ID: GSE29965 Platform: GPL7722 Number of samples: 12 |
| Design and Sample | Cancer Type: esophageal cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: esophageal cancer Control Sample: normal esophagus Num of Case: 4 Num of Control: 4 Quantification Software: Limma Num of miRNAs: 707 |
| Identification | Num of Up: 12 Num of Down: 11 |