| Experiment ID | EXP00161 |
| Reference | Title: The miRNA-200 family and miRNA-9 exhibit differential expression in primaryversus corresponding metastatic tissue in breast cancer. Author: Gravgaard KH, Lyng MB, Laenkholm AV, Søkilde R, Nielsen BS, Litman T, DitzelHJ. Journal: Breast Cancer Res Treat. 2012 Jul;134(1):207-17. doi: 10.1007/s10549-012-1969-9. Abstract: Metastases are the major cause of cancer-related deaths, but the mechanisms ofthe metastatic process remain poorly understood. In recent years, the involvementof microRNAs (miRNAs) in cancer has become apparent, and the objective of thisstudy was to identify miRNAs associated with breast cancer progression. GlobalmiRNA expression profiling was performed on 47 tumor samples from 14 patientswith paired samples from primary breast tumors and corresponding lymph node anddistant metastases using LNA-enhanced miRNA microarrays. The identified miRNAexpression alterations were validated by real-time PCR, and tissue distributionof the miRNAs was visualized by in situ hybridization. The patients, in which themiRNA profile of the primary tumor and corresponding distant metastasis clusteredin the unsupervised cluster analysis, showed significantly shorter intervalsbetween the diagnosis of the primary tumor and distant metastasis (median 1.6years) compared to those that did not cluster (median 11.3 years) (p<0.003).Fifteen miRNAs were identified that were significantly differentially expressedbetween primary tumors and corresponding distant metastases, including miR-9,miR-219-5p and four of the five members of the miR-200 family involved inepithelial-mesenchymal transition. Tumor expression of miR-9 and miR-200b wereconfirmed using in situ hybridization, which also verified higher expression ofthese miRNAs in the distant metastases versus corresponding primary tumors. Ourresults demonstrate alterations in miRNA expression at different stages ofdisease progression in breast cancer, and suggest a direct involvement of themiR-200 family and miR-9 in the metastatic process. PMID: 22294488 |
| Expressiion Profile | Description: The miRNA-200 family and miRNA-9 exhibit differential expression in primary vs. corresponding metastatic tissue in breast cancer Organism: Homo sapiens GEO ID: GSE37407 Platform: GPL13703 Number of samples: 61 |
| Design and Sample | Cancer Type: breast cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: metastasis Case Sample: metastatic breast cancer Control Sample: primary breast cancer Num of Case: 31 Num of Control: 30 Quantification Software: Limma Num of miRNAs: 834 |
| Identification | Num of Up: 72 Num of Down: 86 |