| Experiment ID | EXP00162 |
| Reference | Title: MicroRNA profiling of follicular lymphoma identifies microRNAs related to cellproliferation and tumor response. Author: Wang W, Corrigan-Cummins M, Hudson J, Maric I, Simakova O, Neelapu SS, KwakLW, Janik JE, Gause B, Jaffe ES, Calvo KR. Journal: Haematologica. 2012 Apr;97(4):586-94. doi: 10.3324/haematol.2011.048132. Epub2011 Nov 18. Abstract: BACKGROUND: MicroRNAs can play an important role in tumorigenesis throughpost-transcriptional regulation of gene expression, and are not wellcharacterized in follicular lymphoma.DESIGN AND METHODS: MicroRNA profiles of enriched follicular lymphoma tumor cellsfrom 16 patients were generated by assaying 851 human microRNAs. Tandem geneexpression profiles were obtained for predicting microRNA targets.RESULTS: The expression of 133 microRNAs was significantly different (> 2-fold;P<0.05) between follicular lymphoma and follicular hyperplasia. Forty-fourmicroRNAs in three groups generated a unique follicular lymphoma signature. Ofthese, ten microRNAs were increased (miR-193a-5p, -193b*, -345, -513b, -574-3p,-584, -663, -1287, -1295, and -1471), 11 microRNAs were decreased (miR-17*, -30a,-33a, -106a*, -141, -202, -205, -222, -301b, -431*, and -570), and 23 microRNAsformed a group that was increased in most cases of follicular lymphoma but showedlower expression in a subset of cases (let-7a, let-7f, miR-7-1*, -9, -9*, -20a,-20b, -30b, -96, -98, -194, -195, -221*, -374a, -374b, -451, -454, -502-3p,-532-3p, -664*, -1274a, -1274b, and -1260). Higher expression of this last group was associated with improved response to chemotherapy. Gene expression analysisrevealed increased expression of MAPK1, AKT1, PRKCE, IL4R and DROSHA anddecreased expression of CDKN1A/p21, SOCS2, CHEK1, RAD51, KLF4, BLIMP1 and IRF4 infollicular lymphoma. Functional studies indicated that CDKN1A/p21 and SOCS2expression is directly regulated by miR-20a/-20b and miR-194, respectively.CONCLUSIONS: Follicular lymphoma is characterized by a unique microRNA signature,containing a subset of microRNAs whose expression correlate with response tochemotherapy. miR-20a/b and miR-194 target CDKN1A and SOCS2 in follicularlymphoma, potentially contributing to tumor cell proliferation and survival. PMID: 22102710 |
| Expressiion Profile | Description: microRNA profiling of follicular lymphoma cells Organism: Homo sapiens GEO ID: GSE28090 Platform: GPL11487 Number of samples: 25 |
| Design and Sample | Cancer Type: lymphoma Cancer SubType: B cell lymphoma Cell Line: N/D Experimental Design: cancer vs normal Case Sample: follicular lymphoma Control Sample: follicular hyperplasia Num of Case: 18 Num of Control: 7 Quantification Software: Limma Num of miRNAs: 851 |
| Identification | Num of Up: 56 Num of Down: 32 |