| Experiment ID | EXP00167 |
| Reference | Title: Altered microRNA expression associated with chromosomal changes contributes tocervical carcinogenesis. Author: Wilting SM, Snijders PJ, Verlaat W, Jaspers A, van de Wiel MA, van WieringenWN, Meijer GA, Kenter GG, Yi Y, le Sage C, Agami R, Meijer CJ, Steenbergen RD. Journal: Oncogene. 2013 Jan 3;32(1):106-16. doi: 10.1038/onc.2012.20. Epub 2012 Feb 13. Abstract: Little is known about the alterations in microRNA (miRNA) expression patternsduring the consecutive stages of cervical cancer development and theirassociation with chromosomal instability. In this study, miRNA expression innormal cervical squamous epithelium, high-grade precancerous lesions (cervicalintraepithelial neoplasia (CIN2-3)), squamous cell carcinomas (SCCs) andadenocarcinomas (AdCAs) was integrated with previously generated chromosomalprofiles of the same samples. Significantly differential expression during theconsecutive stages of cervical SCC development was observed for 106 miRNAs. Ofthese differentially expressed miRNAs, 27 showed early transiently alteredexpression in CIN2-3 lesions only, 46 miRNAs showed late altered expression inSCCs only and 33 showed continuously altered expression in both CIN2-3 and SCCs. Altered expression of five significantly differentially expressed miRNAs,hsa-miR-9 (1q23.2), hsa-miR-15b (3q25.32), hsa-miR-28-5p (3q27.3), hsa-miR-100and hsa-miR-125b (both 11q24.1), was directly linked to frequent chromosomalalterations. Functional analyses were performed for hsa-miR-9, representing apotential oncogene with increased expression linked to a chromosomal gain of 1q. Hsa-miR-9 overexpression was found to increase cell viability,anchorage-independent growth and migration in vitro. Upon organic raft culturing,hsa-miR-9 hampered differentiation and induced proliferation in all strata of theepithelial layer. These findings support a potential oncogenic function ofhsa-miR-9 in cervical cancer. In summary, differential expression of 106 miRNAs, partly associated with chromosomal alterations, was observed during cervical SCC development. Altered expression of hsa-miR-9 associated with a chromosomal gainof chromosome 1q was shown to be functionally relevant, underlining theimportance of deregulated miRNA expression in cervical carcinogenesis. PMID: 22330141 |
| Expressiion Profile | Description: Altered microRNA expression associated with chromosomal and epigenetic changes contributes to cervical carcinogenesis Organism: Homo sapiens GEO ID: GSE30656 Platform: GPL6955 Number of samples: 47 |
| Design and Sample | Cancer Type: cervical cancer Cancer SubType: cervical squamous cell carcinoma Cell Line: N/D Experimental Design: cancer vs normal Case Sample: cervical squamous cell carcinoma Control Sample: normal cervical epithelium Num of Case: 10 Num of Control: 10 Quantification Software: Limma Num of miRNAs: 212 |
| Identification | Num of Up: 28 Num of Down: 25 |