| Experiment ID | EXP00176 |
| Reference | Title: Increased miR-708 expression in NSCLC and its association with poor survival inlung adenocarcinoma from never smokers. Author: Jang JS, Jeon HS, Sun Z, Aubry MC, Tang H, Park CH, Rakhshan F, Schultz DA,Kolbert CP, Lupu R, Park JY, Harris CC, Yang P, Jen J. Journal: Clin Cancer Res. 2012 Jul 1;18(13):3658-67. doi: 10.1158/1078-0432.CCR-11-2857.Epub 2012 May 9. Abstract: PURPOSE: miRNA plays an important role in human disease and cancer. We seek toinvestigate the expression status, clinical relevance, and functional role ofmiRNA in non-small cell lung cancer.EXPERIMENTAL DESIGN: We conducted miRNA expression profiling in matched lungadenocarcinoma and uninvolved lung using 56 pairs of fresh-frozen (FF) and 47pairs of formalin-fixed, paraffin-embedded (FFPE) samples from never smokers. Themost differentially expressed miRNA genes were evaluated by Cox analysis andlog-rank test. Among the best candidate, miR-708 was further examined fordifferential expression in two independent cohorts. Functional significance ofmiR-708 expression in lung cancer was examined by identifying its candidate mRNA target and through manipulating its expression levels in cultured cells.RESULTS: Among the 20 miRNAs most differentially expressed between tested tumorand normal samples, high expression level of miR-708 in the tumors was moststrongly associated with an increased risk of death after adjustments for allclinically significant factors including age, sex, and tumor stage (FF cohort:HR, 1.90; 95% CI, 1.08-3.35; P = 0.025 and FFPE cohort: HR, 1.93; 95% CI,1.02-3.63; P = 0.042). The transcript for TMEM88 gene has a miR-708 binding site in its 3' UTR and was significantly reduced in tumors high of miR-708. ForcedmiR-708 expression reduced TMEM88 transcript levels and increased the rate ofcell proliferation, invasion, and migration in culture.CONCLUSIONS: miRNA-708 acts as an oncogene contributing to tumor growth anddisease progression by directly downregulating TMEM88, a negative regulator ofthe Wnt signaling pathway in lung cancer. PMID: 22573352 |
| Expressiion Profile | Description: Increased miR-708 expression in NSCLC and its association with poor survival in lung adenocarcinoma from Never Smokers Organism: Homo sapiens GEO ID: GSE36681 Platform: GPL8179 Number of samples: 206 |
| Design and Sample | Cancer Type: lung cancer Cancer SubType: lung adenocarcinoma Cell Line: N/D Experimental Design: cancer vs normal Case Sample: lung adenocarcinoma Control Sample: normal lung Num of Case: 103 Num of Control: 103 Quantification Software: Limma Num of miRNAs: 846 |
| Identification | Num of Up: 139 Num of Down: 169 |