| Experiment ID | EXP00177 |
| Reference | Title: Keratinization-associated miR-7 and miR-21 regulate tumor suppressorreversion-inducing cysteine-rich protein with kazal motifs (RECK) in oral cancer. Author: Jung HM, Phillips BL, Patel RS, Cohen DM, Jakymiw A, Kong WW, Cheng JQ, ChanEK. Journal: J Biol Chem. 2012 Aug 24;287(35):29261-72. doi: 10.1074/jbc.M112.366518. Epub2012 Jul 2. Abstract: MicroRNAs (miRNAs) are small non-coding RNAs that posttranscriptionally regulate gene expression during many biological processes. Recently, the aberrantexpressions of miRNAs have become a major focus in cancer research. The purposeof this study was to identify deregulated miRNAs in oral cancer and further focuson specific miRNAs that were related to patient survival. Here, we report thatmiRNA expression profiling provided more precise information when oral squamouscell carcinomas were subcategorized on the basis of clinicopathologicalparameters (tumor primary site, histological subtype, tumor stage, and HPV16status). An innovative radar chart analysis method was developed to depictsubcategories of cancers taking into consideration the expression patterns ofmultiple miRNAs combined with the clinicopathological parameters. Keratinization of tumors and the high expression of miR-21 were the major factors related to thepoor prognosis of patients. Interestingly, a majority of the keratinized tumorsexpressed high levels of miR-21. Further investigations demonstrated theregulation of the tumor suppressor gene reversion-inducing cysteine-rich protein with kazal motifs (RECK) by two keratinization-associated miRNAs, miR-7 andmiR-21. Transfection of miR-7 and miR-21-mimics reduced the expression of RECKthrough direct miRNA-mediated regulation, and these miRNAs were inverselycorrelated with RECK in CAL 27 orthotopic xenograft tumors. Furthermore, asimilar inverse correlation was demonstrated in CAL 27 cells treated in vitro by different external stimuli such as trypsinization, cell density, and serumconcentration. Taken together, our data show that keratinization is associatedwith poor prognosis of oral cancer patients and keratinization-associated miRNAs mediate deregulation of RECK which may contribute to the aggressiveness oftumors. PMID: 22761427 |
| Expressiion Profile | Description: Differential expression of microRNAs between oral squamous cell carcinoma and healthy control tongues Organism: Homo sapiens GEO ID: GSE28100 Platform: GPL10850 Number of samples: 20 |
| Design and Sample | Cancer Type: oral squamous cell carcinoma Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: oral squamous cell carcinoma Control Sample: healthy control tongue Num of Case: 17 Num of Control: 3 Quantification Software: Limma Num of miRNAs: 851 |
| Identification | Num of Up: 37 Num of Down: 20 |