| Experiment ID | EXP00178 |
| Reference | Title: An integrated analysis of miRNA and mRNA expressions in non-small cell lungcancers. Author: Ma L, Huang Y, Zhu W, Zhou S, Zhou J, Zeng F, Liu X, Zhang Y, Yu J. Journal: PLoS One. 2011;6(10):e26502. doi: 10.1371/journal.pone.0026502. Epub 2011 Oct 27. Abstract: Using DNA microarrays, we generated both mRNA and miRNA expression data from 6non-small cell lung cancer (NSCLC) tissues and their matching normal control fromadjacent tissues to identify potential miRNA markers for diagnostics. Wedemonstrated that hsa-miR-96 is significantly and consistently up-regulated inall 6 NSCLCs. We validated this result in an independent set of 35 paired tumors and their adjacent normal tissues, as well as their sera that are collectedbefore surgical resection or chemotherapy, and the results suggested thathsa-miR-96 may play an important role in NSCLC development and has greatpotential to be used as a noninvasive marker for diagnosing NSCLC. We predictedpotential miRNA target mRNAs based on different methods (TargetScan and miRanda).Further classification of miRNA regulated genes based on their relationship with miRNAs revealed that hsa-miR-96 and certain other miRNAs tend to down-regulatetheir target mRNAs in NSCLC development, which have expression levels permissive to direct interaction between miRNAs and their target mRNAs. In addition, weidentified a significant correlation of miRNA regulation with genes coincide withhigh density of CpG islands, which suggests that miRNA may represent a primaryregulatory mechanism governing basic cellular functions and celldifferentiations, and such mechanism may be complementary to DNA methylation inrepressing or activating gene expression. PMID: 22046296 |
| Expressiion Profile | Description: Genome-wide analysis of microRNA expression in Non-small Cell Lung Cancer Organism: Homo sapiens GEO ID: GSE29248 Platform: GPL8179 Number of samples: 12 |
| Design and Sample | Cancer Type: lung cancer Cancer SubType: lung squamous cell carcinoma Cell Line: N/D Experimental Design: cancer vs normal Case Sample: lung squamous cell carcinoma Control Sample: normal lung Num of Case: 3 Num of Control: 3 Quantification Software: Limma Num of miRNAs: 857 |
| Identification | Num of Up: 1 Num of Down: 0 |