| Experiment ID | EXP00182 |
| Reference | Title: MiRNA expression analysis of cancer-associated fibroblasts and normal fibroblastsin breast cancer. Author: Zhao L, Sun Y, Hou Y, Peng Q, Wang L, Luo H, Tang X, Zeng Z, Liu M. Journal: Int J Biochem Cell Biol. 2012 Nov;44(11):2051-9. doi:10.1016/j.biocel.2012.08.005. Epub 2012 Aug 13. Abstract: Cancer-associated fibroblasts (CAFs) promote tumorigenesis, growth, invasion and metastasis of cancer, whereas normal fibroblasts (NFs) are thought to suppresstumor progression. Little is known about miRNAs expression differences betweenCAFs and NFs or the patient-to-patient variability in miRNAs expression in breastcancer. We established primary cultures of CAFs and paired NFs from six resected breast tumor tissues that had not previously received radiotherapy orchemotherapy treatment and analyzed with miRNAs microarrays. The array data were analyzed using paired SAM t-test and filtered according to α and q values.Pathway analysis was conducted using DAVID v6.7. We identified 11 dysregulatedmiRNAs in CAFs: three were up-regulated (miR-221-5p, miR-31-3p, miR-221-3p),while eight were down-regulated (miR-205, miR-200b, miR-200c, miR-141, miR-101,miR-342-3p, let-7g, miR-26b). Their target genes are known to affect celldifferentiation, adhesion, migration, proliferation, secretion and cell-cellinteraction. By our knowledge it is firstly identify the expression profiles ofmiRNAs between CAFs and NFs and revealed their regulation on the associatedsignaling pathways. PMID: 22964023 |
| Expressiion Profile | Description: MiRNA expression analysis of cancer-associated fibroblasts (CAFs) and normal fibroblasts (NFs) in breast cancer Organism: Homo sapiens GEO ID: GSE37527 Platform: GPL14767 Number of samples: 12 |
| Design and Sample | Cancer Type: breast cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: breast cancer fibroblast Control Sample: normal fibroblast Num of Case: 6 Num of Control: 6 Quantification Software: Limma Num of miRNAs: 851 |
| Identification | Num of Up: 2 Num of Down: 5 |