| Experiment ID | EXP00184 |
| Reference | Title: The rectal cancer microRNAome--microRNA expression in rectal cancer and matchednormal mucosa. Author: Gaedcke J, Grade M, Camps J, Søkilde R, Kaczkowski B, Schetter AJ,Difilippantonio MJ, Harris CC, Ghadimi BM, Møller S, Beissbarth T, Ried T, LitmanT. Journal: Clin Cancer Res. 2012 Sep 15;18(18):4919-30. Epub 2012 Jul 31. Abstract: PURPOSE: miRNAs play a prominent role in a variety of physiologic and pathologic biologic processes, including cancer. For rectal cancers, only limited data areavailable on miRNA expression profiles, whereas the underlying genomic andtranscriptomic aberrations have been firmly established. We therefore, aimed tocomprehensively map the miRNA expression patterns of this disease.EXPERIMENTAL DESIGN: Tumor biopsies and corresponding matched mucosa samples wereprospectively collected from 57 patients with locally advanced rectal cancers.Total RNA was extracted, and tumor and mucosa miRNA expression profiles weresubsequently established for all patients. The expression of selected miRNAs was validated using semi-quantitative real-time PCR.RESULTS: Forty-nine miRNAs were significantly differentially expressed(log(2)-fold difference >0.5 and P < 0.001) between rectal cancer and normalrectal mucosa. The predicted targets for these miRNAs were enriched for thefollowing pathways: Wnt, TGF-beta, mTOR, insulin, mitogen-activated proteinkinase, and ErbB signaling. Thirteen of these 49 miRNAs seem to be rectalcancer-specific, and have not been previously reported for colon cancers:miR-492, miR-542-5p, miR-584, miR-483-5p, miR-144, miR-2110, miR-652, miR-375,miR-147b, miR-148a, miR-190, miR-26a/b, and miR-338-3p. Of clinical impact,miR-135b expression correlated significantly with disease-free andcancer-specific survival in an independent multicenter cohort of 116 patients.CONCLUSION: This comprehensive analysis of the rectal cancer miRNAome uncoverednovel miRNAs and pathways associated with rectal cancer. This informationcontributes to a detailed view of this disease. Moreover, the identification and validation of miR-135b may help to identify novel molecular targets and pathways for therapeutic exploitation. PMID: 22850566 |
| Expressiion Profile | Description: The rectal cancer microRNAome - a comprehensive comparative analysis of microRNA expression in rectal cancer and matched normal mucosa Organism: Homo sapiens GEO ID: GSE38389 Platform: GPL11039 Number of samples: 140 |
| Design and Sample | Cancer Type: colorectal cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: rectal tumor Control Sample: normal trectal mucosa Num of Case: 69 Num of Control: 71 Quantification Software: Limma Num of miRNAs: 581 |
| Identification | Num of Up: 137 Num of Down: 130 |