| Experiment ID | EXP00195 |
| Reference | Title: miR-10b*, a master inhibitor of the cell cycle, is down-regulated in human breasttumours. Author: Biagioni F, Bossel Ben-Moshe N, Fontemaggi G, Canu V, Mori F, Antoniani B, Di Benedetto A, Santoro R, Germoni S, De Angelis F, Cambria A, Avraham R, Grasso G, Strano S, Muti P, Mottolese M, Yarden Y, Domany E, Blandino G. Journal: EMBO Mol Med. 2012 Nov;4(11):1214-29. doi: 10.1002/emmm.201201483. Abstract: Deregulated proliferation is a hallmark of cancer cells. Here, we show thatmicroRNA-10b* is a master regulator of breast cancer cell proliferation and isdownregulated in tumoural samples versus matched peritumoural counterparts. Twocanonical CpG islands (5 kb) upstream from the precursor sequence arehypermethylated in the analysed breast cancer tissues. Ectopic delivery ofsynthetic microRNA-10b* in breast cancer cell lines or into xenograft mousebreast tumours inhibits cell proliferation and impairs tumour growth in vivo,respectively. We identified and validated in vitro and in vivo three novel targetmRNAs of miR-10b* (BUB1, PLK1 and CCNA2), which play a remarkable role in cellcycle regulation and whose high expression in breast cancer patients isassociated with reduced disease-free survival, relapse-free survival andmetastasis-free survival when compared to patients with low expression. This alsosuggests that restoration of microRNA-10b* expression might have therapeuticpromise. PMID: 23125021 |
| Expressiion Profile | Description: miR-10b*, a master inhibitor of the cell cycle, is downregulated in human breast tumors Organism: Homo sapiens GEO ID: GSE40525 Platform: GPL8227 Number of samples: 120 |
| Design and Sample | Cancer Type: breast cancer Cancer SubType: ER negative Cell Line: N/D Experimental Design: cancer vs normal Case Sample: ER negative breast tumour Control Sample: normal tissue Num of Case: 14 Num of Control: 59 Quantification Software: Limma Num of miRNAs: 723 |
| Identification | Num of Up: 115 Num of Down: 113 |