| Experiment ID | EXP00201 |
| Reference | Title: Comparative microarray analysis of microRNA expression profiles in primarycutaneous malignant melanoma, cutaneous malignant melanoma metastases, and benignmelanocytic nevi. Author: Sand M, Skrygan M, Sand D, Georgas D, Gambichler T, Hahn SA, Altmeyer P,Bechara FG. Journal: Cell Tissue Res. 2013 Jan;351(1):85-98. doi: 10.1007/s00441-012-1514-5. Epub 2012Oct 31. Abstract: Perturbations in microRNA (miRNA) expression profiles have been reported forcutaneous malignant melanoma (CMM) predominantly when examined in cell lines.Despite the rapidly growing number of newly discovered human miRNA sequences, theavailability of up-to-date miRNA expression profiles for clinical samples ofprimary cutaneous malignant melanoma (PCMM), cutaneous malignant melanomametastases (CMMM), and benign melanocytic nevi (BMN) is limited. Specimensexcised from the center of tumors (lesional) from patients with PCMM (n=9), CMMM (n=4), or BMN (n=8) were obtained during surgery. An exploratory microarrayanalysis was performed by miRNA expression profiling based on Agilent platformscreening for 1205 human miRNAs. The results from the microarray analysis werevalidated by TaqMan quantitative real-time polymerase chain reaction. In additionto several miRNAs previously known to be associated with CMM, 19 unidentifiedmiRNA candidates were found to be dysregulated in CMM patient samples. Among the 19 novel miRNA candidates, the genes hsa-miR-22, hsa-miR-130b, hsa-miR-146b-5p,hsa-miR-223, hsa-miR-301a, hsa-miR-484, hsa-miR-663, hsa-miR-720, hsa-miR-1260,hsa-miR-1274a, hsa-miR-1274b, hsa-miR-3663-3p, hsa-miR-4281, and hsa-miR-4286were upregulated, and the genes hsa-miR-24-1*, hsa-miR-26a, hsa-miR-4291,hsa-miR-4317, and hsa-miR-4324 were downregulated. The results of this studypartially confirm previous CMM miRNA profiling studies identifying miRNAs thatare dysregulated in CMM. However, we report several novel miRNA candidates in CMMtumors; these miRNA sequences require further validation and functional analysis to evaluate whether they play a role in the pathogenesis of CMM. PMID: 23111773 |
| Expressiion Profile | Description: Comparative microarray analysis of microRNA expression profiles in primary cutaneous malignant melanoma, cutaneous malignant melanoma metastases and benign melanocytic naevi Organism: Homo sapiens GEO ID: GSE34460 Platform: GPL15019 Number of samples: 21 |
| Design and Sample | Cancer Type: melanoma Cancer SubType: N/D Cell Line: N/D Experimental Design: metastasis Case Sample: metastatic melanoma Control Sample: primary melanoma Num of Case: 4 Num of Control: 9 Quantification Software: Limma Num of miRNAs: 1205 |
| Identification | Num of Up: 3 Num of Down: 0 |