| Experiment ID | EXP00204 |
| Reference | Title: The isolation and characterization of renal cancer initiating cells from humanWilms' tumour xenografts unveils new therapeutic targets. Author: Pode-Shakked N, Shukrun R, Mark-Danieli M, Tsvetkov P, Bahar S, Pri-Chen S,Goldstein RS, Rom-Gross E, Mor Y, Fridman E, Meir K, Simon A, Magister M,Kaminski N, Goldmacher VS, Harari-Steinberg O, Dekel B. Journal: EMBO Mol Med. 2013 Jan;5(1):18-37. doi: 10.1002/emmm.201201516. Epub 2012 Dec 13. Abstract: There are considerable differences in tumour biology between adult and paediatriccancers. The existence of cancer initiating cells/cancer stem cells (CIC/CSC) in paediatric solid tumours is currently unclear. Here, we show the successfulpropagation of primary human Wilms' tumour (WT), a common paediatric renalmalignancy, in immunodeficient mice, demonstrating the presence of a populationof highly proliferative CIC/CSCs capable of serial xenograft initiation. Cellsorting and limiting dilution transplantation analysis of xenograft cellsidentified WT CSCs that harbour a primitive undifferentiated-NCAM1expressing-blastema phenotype, including a capacity to expand and differentiateinto the mature renal-like cell types observed in the primary tumour. WT CSCs,which can be further enriched by aldehyde dehydrogenase activity, overexpressedrenal stemness and genes linked to poor patient prognosis, showed preferentialprotein expression of phosphorylated PKB/Akt and strong reduction of the miR-200 family. Complete eradication of WT in multiple xenograft models was achieved witha human NCAM antibody drug conjugate. The existence of CIC/CSCs in WT providesnew therapeutic targets. PMID: 23239665 |
| Expressiion Profile | Description: Prospective isolation and characterization of renal cancer stem cells from human Wilms' tumor xenografts leads to tumor eradication Organism: Homo sapiens GEO ID: GSE33332 Platform: GPL10850 Number of samples: 12 |
| Design and Sample | Cancer Type: kidney cancer Cancer SubType: Wilms tumor Cell Line: N/D Experimental Design: cancer vs normal Case Sample: Wilms tumor Control Sample: fetal kidney Num of Case: 4 Num of Control: 4 Quantification Software: Limma Num of miRNAs: 851 |
| Identification | Num of Up: 23 Num of Down: 28 |