Experiment ID | EXP00205 |
Reference | Title: Targeting glioma stem cells by functional inhibition of a prosurvival oncomiR-138in malignant gliomas. Author: Chan XH, Nama S, Gopal F, Rizk P, Ramasamy S, Sundaram G, Ow GS, Ivshina AV,Tanavde V, Haybaeck J, Kuznetsov V, Sampath P. Journal: Cell Rep. 2012 Sep 27;2(3):591-602. doi: 10.1016/j.celrep.2012.07.012. Epub 2012 Aug 24. Abstract: Malignant gliomas are the most aggressive forms of brain tumors, associated with high rates of morbidity and mortality. Recurrence and tumorigenesis areattributed to a subpopulation of tumor-initiating glioma stem cells (GSCs) thatare intrinsically resistant to therapy. Initiation and progression of gliomashave been linked to alterations in microRNA expression. Here, we report theidentification of microRNA-138 (miR-138) as a molecular signature of GSCs anddemonstrate a vital role for miR-138 in promoting growth and survival of bonafide tumor-initiating cells with self-renewal potential. Sequence-specificfunctional inhibition of miR-138 prevents tumorsphere formation in vitro andimpedes tumorigenesis in vivo. We delineate the components of the miR-138regulatory network by loss-of-function analysis to identify specific regulatorsof apoptosis. Finally, the higher expression of miR-138 in GSCs compared tonon-neoplastic tissue and association with tumor recurrence and survivalhighlights the clinical significance of miR-138 as a prognostic biomarker and atherapeutic target for treatment of malignant gliomas. PMID: 22921398 |
Expressiion Profile | Description: MicroRNA-138: A Pro-survival OncomiR for Glioma Stem Cells is Associated with Tumor Recurrence [miRNA] Organism: Homo sapiens GEO ID: GSE39486 Platform: GPL15829 Number of samples: 6 |
Design and Sample | Cancer Type: brain cancer Cancer SubType: glioma Cell Line: N/D Experimental Design: cancer vs normal Case Sample: glioma Control Sample: normal brain Num of Case: 3 Num of Control: 3 Quantification Software: Limma Num of miRNAs: 341 |
Identification | Num of Up: 67 Num of Down: 71 |