| Experiment ID | EXP00213 |
| Reference | Title: Identification of microRNAs specifically expressed in hepatitis Cvirus-associated hepatocellular carcinoma. Author: Diaz G, Melis M, Tice A, Kleiner DE, Mishra L, Zamboni F, Farci P. Journal: Int J Cancer. 2013 Aug 15;133(4):816-24. doi: 10.1002/ijc.28075. Epub 2013 Mar16. Abstract: Although several studies have investigated the association of miRNAs withhepatocellular carcinoma (HCC), the data published so far are not concordant. Areason for these discrepancies may be the fact that most studies used thenontumorous tissue surrounding the HCC lesion as a control, which is almostinvariably affected by cirrhosis or chronic hepatitis, as well as otherpathological conditions such as hepatitis B virus (HBV) or hepatitis C virus(HCV) infection. Moreover, HCC is often analyzed as a single group regardless of the different viral etiologies. The miRNAs differentially expressed inHCV-related HCC were investigated by comparing the tumorous tissues to a widerange of liver specimens, including healthy livers obtained from liver donors andpatients who underwent liver resection for angioma, in addition to tissues fromvarious acute and chronic liver diseases, including HCV-related cirrhosis notassociated with HCC, HCV-related cirrhosis associated with HCC and HBV-associatedacute liver failure. The whole set of 2,226 human miRNAs were examined, including1,121 pre-miRNAs and 1,105 mature miRNAs, available in a microarray platform.Stringent statistical methods were applied to reduce the risk of falsediscoveries to less than 1%. These data identified 18 miRNAs exclusivelyexpressed in HCV-associated HCC, characterized by high specificity andselectivity versus all other liver diseases and healthy conditions and connected into a regulatory network pivoting on p53, phosphatase and tensin homolog andall-trans retinoic acid signaling. PMID: 23390000 |
| Expressiion Profile | Description: Identification of microRNAs specifically expressed in HCV-associated hepatocellular carcinoma Organism: Homo sapiens GEO ID: GSE40744 Platform: GPL14613 Number of samples: 76 |
| Design and Sample | Cancer Type: hepatocellular carcinoma Cancer SubType: N/D Cell Line: N/D Experimental Design: cancer vs normal Case Sample: HCV-associated HCC Control Sample: normal liver Num of Case: 26 Num of Control: 7 Quantification Software: Limma Num of miRNAs: 1105 |
| Identification | Num of Up: 48 Num of Down: 64 |