| Experiment ID | EXP00223 |
| Reference | Title: The expression level of miR-18b in hepatocellular carcinoma is associated withthe grade of malignancy and prognosis. Author: Murakami Y, Tamori A, Itami S, Tanahashi T, Toyoda H, Tanaka M, Wu W, BrojiginN, Kaneoka Y, Maeda A, Kumada T, Kawada N, Kubo S, Kuroda M. Journal: BMC Cancer. 2013 Mar 4;13:99. doi: 10.1186/1471-2407-13-99. Abstract: BACKGROUND: Many studies support the hypothesis that specific microRNA (miRNA)expression in various human cancers including hepatocarcinogenesis is closelyassociated with diagnosis and prognosis. In hepatocellular carcinoma (HCC),malignancy level is related to the degree of histological differentiation.METHODS: In order to establish a novel biomarker that can determine the degree ofmalignancy and forecast patient prognosis, we performed a microarray analysis to investigate the miRNA expression profiles in 110 HCC which were comprised of 60moderately, 30 poorly, and 20 well differentiated HCC.RESULTS: We found that the expression of 12 miRNAs varied significantly accordingto the degree of histological differentiation. Particularly, miR-18b expressionin poorly differentiated HCC was significantly higher than in well differentiatedHCC. Based on miRanda and Targetscan target search algorithms and Argonaute 2immunoprecipitation study, we noted that miR-18b can control the expression oftrinucleotide repeat containing 6B (TNRC6B) as a target gene. Additionally, intwo hepatoma cell lines, we found that over-expression of miR-18b ordown-regulation of TNRC6B accelerated cell proliferation and loss of celladhesion ability. Finally, we observed that after surgical resection, HCCpatients with high miR-18b expression had a significantly shorter relapse-freeperiod than those with low expression.CONCLUSIONS: miR-18b expression is an important marker of cell proliferation and cell adhesion, and is predictive of clinical outcome. From a clinical point ofview, our study emphasizes miR-18b as a diagnostic and prognostic marker for HCC progression. PMID: 23496901 |
| Expressiion Profile | Description: Over-expression of miR-18b is associated with the grade of malignancy of hepatocallular carcinoma Organism: Homo sapiens GEO ID: GSE31164 Platform: GPL10850 Number of samples: 110 |
| Design and Sample | Cancer Type: hepatocellular carcinoma Cancer SubType: N/D Cell Line: N/D Experimental Design: high grade vs low grade Case Sample: hepatocellular carcinoma poor differentiation Control Sample: hepatocellular carcinoma moderate differentiation Num of Case: 30 Num of Control: 60 Quantification Software: Limma Num of miRNAs: 851 |
| Identification | Num of Up: 15 Num of Down: 7 |