| Experiment ID | EXP00226 |
| Reference | Title: Direct targeting of SUZ12/ROCK2 by miR-200b/c inhibits cholangiocarcinomatumourigenesis and metastasis. Author: Peng F, Jiang J, Yu Y, Tian R, Guo X, Li X, Shen M, Xu M, Zhu F, Shi C, Hu J, Wang M, Qin R. Journal: Br J Cancer. 2013 Dec 10;109(12):3092-104. doi: 10.1038/bjc.2013.655. Epub 2013Oct 29. Abstract: BACKGROUND: The multidrug resistance and distant metastasis of cholangiocarcinomaresult in high postoperative recurrence and low long-term survival rates. It has been demonstrated that the ectopic expression of miR-200 suppresses the multidrugresistance and metastasis of cancer. However, the expression and function ofmiR-200 in cholangiocarcinoma has not yet been described.METHODS: In this study, we identified dysregulated microRNAs (miRNAs, miR) incholangiocarcinoma tissue by microarray analysis, and subsequent real-time PCRand northern blot analyses validated the expression of candidate miR. Weperformed functional analyses and investigated the relationship betweenmiR-200b/c expression and the properties of cholangiocarcinoma cells. A dualluciferase assay was applied to examine the effect of miRNAs on the 3'-UTR oftarget genes, and we demonstrated the function of the target gene by siRNAtransfection identifying the downstream pathway via western blotting.RESULTS: We found significantly downregulated expression of four miR-200 familymembers (miR-200a/b/c/429) and then confirmed that ectopic miR-200b/200c inhibitsthe migration and invasion of cholangiocarcinoma cells both in vitro and in vivo.We found that miR-200b/c influenced the tumourigenesis of cholangiocarcinomacells including their tumour-initiating capacity, sphere formation, and drugresistance. We further found that miR-200b/c regulated migration and invasioncapacities by directly targeting rho-kinase 2 and regulated tumorigenicproperties by directly targeting SUZ12 (a subunit of a polycomb repressorcomplex).CONCLUSION: Our study shows that miR-200b/c has a critical role in the regulationof the tumorigenic and metastatic capacity of cholangiocarcinoma and reveals the probable underlying mechanisms. PMID: 24169343 |
| Expressiion Profile | Description: Cholangiocarcinoma tissues VS. corresponding normal bile duct tissues Organism: Homo sapiens GEO ID: GSE47764 Platform: GPL11487 Number of samples: 6 |
| Design and Sample | Cancer Type: biliary tract cancer Cancer SubType: cholangiocarcinoma Cell Line: N/D Experimental Design: cancer vs normal Case Sample: cholangiocarcinoma Control Sample: normal bile duct tissue Num of Case: 3 Num of Control: 3 Quantification Software: Limma Num of miRNAs: 851 |
| Identification | Num of Up: 0 Num of Down: 1 |