| Experiment ID | EXP00233 |
| Reference | Title: Restricted expression of miR-30c-2-3p and miR-30a-3p in clear cell renal cellcarcinomas enhances HIF2α activity. Author: Mathew LK, Lee SS, Skuli N, Rao S, Keith B, Nathanson KL, Lal P, Simon MC. Journal: Cancer Discov. 2014 Jan;4(1):53-60. doi: 10.1158/2159-8290.CD-13-0291. Epub 2013 Nov 4. Abstract: Inactivation of the von-Hippel Lindau (VHL) tumor suppressor gene occurs in 90%of human clear cell renal cell carcinomas (ccRCC) and leads to the stableexpression of the hypoxia-inducible factors HIF1α and HIF2α. The constitutiveexpression of HIF1α in a majority of VHL-deficient tumors is counterintuitive,given that HIF1α functions as a tumor suppressor in ccRCC, whereas HIF2α clearly enhances tumor growth. We demonstrate here that miR-30c-2-3p and miR-30a-3pspecifically bind and inhibit expression of HIF2A transcripts, and that the locusencoding miR-30c-2-3p and miR-30a-3p is selectively repressed in H1H2VHL-deficient tumors expressing both HIF1α and HIF2α proteins. InhibitingmiR-30a-3p expression increases HIF2α levels in H1H2 ccRCC cells and promotescellular proliferation, angiogenesis, and xenograft tumor growth. Our resultsindicate that miR-30c-2-3p and miR-30a-3p repression enhances HIF2α expressionand suggests a mechanism whereby the tumor-suppressive effects of constitutiveHIF1α expression are attenuated in VHL-deficient H1H2 tumors. PMID: 24189146 |
| Expressiion Profile | Description: miRNA expression in primary renal carcinomas, subgrouped based on HIF1α expression Organism: Homo sapiens GEO ID: GSE47582 Platform: GPL8786 Number of samples: 26 |
| Design and Sample | Cancer Type: kidney cancer Cancer SubType: clear cell renal cell carcinoma Cell Line: N/D Experimental Design: cancer vs normal Case Sample: clear cell renal cell carcinoma Control Sample: normal kidney Num of Case: 13 Num of Control: 13 Quantification Software: Limma Num of miRNAs: 597 |
| Identification | Num of Up: 48 Num of Down: 49 |