| Experiment ID | EXP00234 |
| Reference | Title: miR-1 induces growth arrest and apoptosis in malignant mesothelioma. Author: Xu Y, Zheng M, Merritt RE, Shrager JB, Wakelee H, Kratzke RA, Hoang CD. Journal: Chest. 2013 Nov;144(5):1632-43. doi: 10.1378/chest.12-2770. Abstract: BACKGROUND: We investigated microRNA expression profiles of malignant pleuralmesothelioma (MPM) specimens to identify novel microRNA that are potentiallyinvolved in the oncogenic transformation of human pleural cells.METHODS: microRNA microarray transcriptional profiling studies of 25 MPM primary tumors were performed. We used normal pleural tissue from an unmatched patientcohort as normal comparators. To confirm microarray data, we used real-timequantitative polymerase chain reaction. Representative cell lines H513 and H2052 were used in functional analyses of miR-1.RESULTS: In addition to several novel MPM-associated microRNAs, we observed that the expression level of miR-1 was significantly lower in tumors as compared with normal pleural specimens. Subsequently, pre-miR of miR-1 was introduced into MPM cell lines to overexpress this microRNA. Phenotypic changes of these alteredcells were assayed. The cellular proliferation rate was significantly inhibitedafter overexpression of miR-1. Early and late apoptosis was increased markedly inmiR-1-transfected cell lines. Taken together, these data suggested thatoverexpression of miR-1 induced apoptosis in these MPM cell lines, acting as atumor suppressor. We confirmed our observations by assessing in the transducedMPM cells cell cycle-related, proapoptotic, and antiapoptotic genes, which allshowed coordinated, significant changes characteristic of the apoptoticphenotype.CONCLUSIONS: Further investigation and validation of our microRNA database of MPMmay elucidate previously unrecognized molecular pathways and/or mechanisms byidentifying novel microRNAs that are involved in malignant transformation. Ourstudy has now found miR-1 to be one of these MPM-associated microRNAs, withpotential pathogenic and therapeutic significance. PMID: 23828229 |
| Expressiion Profile | Description: Human Mesothelioma MicroRNA Study Part 1 Organism: Homo sapiens GEO ID: GSE40345 Platform: GPL8179 Number of samples: 31 |
| Design and Sample | Cancer Type: mesothelioma Cancer SubType: malignant pleural mesothelioma Cell Line: N/D Experimental Design: cancer vs normal Case Sample: malignant pleural mesothelioma Control Sample: normal tissue Num of Case: 25 Num of Control: 6 Quantification Software: Limma Num of miRNAs: 846 |
| Identification | Num of Up: 97 Num of Down: 107 |