| Experiment ID | EXP00249 |
| Reference | Title: Characterization of gene expression and activated signaling pathways insolid-pseudopapillary neoplasm of pancreas. Author: Park M, Kim M, Hwang D, Park M, Kim WK, Kim SK, Shin J, ParkES, Kang CM, Paik YK, Kim H. Journal: Mod Pathol. 2014 Apr;27(4):580-93. doi: 10.1038/modpathol.2013.154. Epub 2013 Sep27. Abstract: Solid-pseudopapillary neoplasm is an uncommon pancreatic tumor with distinctclinicopathologic features. Solid-pseudopapillary neoplasms are characterized by mutations in exon 3 of CTNNB1. However, little is known about the gene andmicroRNA expression profiles of solid-pseudopapillary neoplasms. Thus, we sought to characterize solid-pseudopapillary neoplasm-specific gene expression andidentify the signaling pathways activated in these tumors. Comparisons of geneexpression in solid-pseudopapillary neoplasm to pancreatic ductal carcinomas,neuroendocrine tumors, and non-neoplastic pancreatic tissues identifiedsolid-pseudopapillary neoplasm-specific mRNA and microRNA profiles. By analyzing 1686 (1119 upregulated and 567 downregulated) genes differentially expressed insolid-pseudopapillary neoplasm, we found that the Wnt/β-catenin, Hedgehog, andandrogen receptor signaling pathways, as well as genes involved in epithelialmesenchymal transition, are activated in solid-pseudopapillary neoplasms. Wevalidated these results experimentally by assessing the expression of β-catenin, WIF-1, GLI2, androgen receptor, and epithelial-mesenchymal transition-relatedmarkers with western blotting and immunohistochemistry. Our analysis alsorevealed 17 microRNAs, especially the miR-200 family and miR-192/215, closelyassociated with the upregulated genes associated with the three pathwaysactivated in solid-pseudopapillary neoplasm and epithelial mesenchymaltransition. Our results provide insight into the molecular mechanisms underlying solid-pseudopapillary neoplasm tumorigenesis and its characteristic lessepithelial cell differentiation than the other common pancreatic tumors. PMID: 24072181 |
| Expressiion Profile | Description: microRNA expression of pancreatic tumors Organism: Homo sapiens GEO ID: GSE43796 Platform: GPL15159 Number of samples: 31 |
| Design and Sample | Cancer Type: pancreatic cancer Cancer SubType: pancreatic ductal adenocarcinoma Cell Line: N/D Experimental Design: cancer vs normal Case Sample: pancreatic ductal adenocarcinoma Control Sample: normal pancreas Num of Case: 7 Num of Control: 5 Quantification Software: Limma Num of miRNAs: 1205 |
| Identification | Num of Up: 28 Num of Down: 56 |