| Experiment ID | EXP00252 |
| Reference | Title: Complementary strand microRNAs mediate acquisition of metastatic potential incolonic adenocarcinoma. Author: Chen DT, Hernandez JM, Shibata D, McCarthy SM, Humphries LA, Clark W, Elahi A,Gruidl M, Coppola D, Yeatman T. Journal: J Gastrointest Surg. 2012 May;16(5):905-12; discussion 912-3. doi:10.1007/s11605-011-1815-0. Epub 2012 Feb 24. Abstract: BACKGROUND: Altered expression of specific microRNAs (miRNA) is known to occurduring colorectal carcinogenesis. However, little is known about the genome-wide alterations in miRNA expression during the neoplastic progression of primarycolorectal cancers.METHODS: Using a miRNA array platform, we evaluated the expression of 668 miRNAin primary colonic adenocarcinomas. Biological functions of selected miRNA wereevaluated with in vitro invasion assays.RESULTS: RNA was extracted for miRNA analysis from 65 primary colon cancers. Weidentified a seven-miRNA expression signature that differentiated stage I andstage IV primary colon cancers. We then demonstrated this signature was able todiscriminate between stage II and III primary colon cancers. Six differentiallyexpressed miRNA were downregulated in association with the development ofmetastases, and all 7 miRNA were complementary strand miRNA. We transfectedHCT-116, a highly invasive colon cancer cell line, with correspondingdownregulated miRNA and demonstrated that overexpression of three miRNA(miR200c*, miR143*, and miR424*) significantly abrogated invasive potential.CONCLUSION: We have identified a seven-miRNA signature that is associated withmetastatic potential in the primary tumor. Forced overexpression of threedownregulated miRNA resulted in attenuation of in vitro invasion, suggestingdirect tumor suppressive function and further supporting the biologicalimportance of complementary strand miRNA. PMID: 22362069 |
| Expressiion Profile | Description: Complementary Strand MicroRNAs Mediate Acquisition of Metastatic Potential in Colonic AdenocarcinomamRNA and microRNA profile in colon cancer Organism: Homo sapiens GEO ID: GSE29622 Platform: GPL11162 Number of samples: 65 |
| Design and Sample | Cancer Type: colon cancer Cancer SubType: N/D Cell Line: N/D Experimental Design: metastasis Case Sample: metastatic colon cancer Control Sample: primary colon cancer Num of Case: 18 Num of Control: 46 Quantification Software: Limma Num of miRNAs: 577 |
| Identification | Num of Up: 1 Num of Down: 0 |