| Experiment ID | EXP00259 |
| Reference | Title: Global miRNA expression analysis of serous and clear cell ovarian carcinomasidentifies differentially expressed miRNAs including miR-200c-3p as a prognostic marker. Author: Vilming Elgaaen B, Olstad OK, Haug KB, Brusletto B, Sandvik L, Staff AC,Gautvik KM, Davidson B. Journal: BMC Cancer. 2014 Feb 11;14:80. doi: 10.1186/1471-2407-14-80. Abstract: BACKGROUND: Improved insight into the molecular characteristics of the different ovarian cancer subgroups is needed for developing a more individualized andoptimized treatment regimen. The aim of this study was to a) identifydifferentially expressed miRNAs in high-grade serous ovarian carcinoma (HGSC),clear cell ovarian carcinoma (CCC) and ovarian surface epithelium (OSE), b)evaluate selected miRNAs for association with clinical parameters includingsurvival and c) map miRNA-mRNA interactions.METHODS: Differences in miRNA expression between HGSC, CCC and OSE were analyzed by global miRNA expression profiling (Affymetrix GeneChip miRNA 2.0 Arrays, n =12, 9 and 9, respectively), validated by RT-qPCR (n = 35, 19 and 9,respectively), and evaluated for associations with clinical parameters. For HGSC,differentially expressed miRNAs were linked to differentially expressed mRNAsidentified previously.RESULTS: Differentially expressed miRNAs (n = 78) between HGSC, CCC and OSE were identified (FDR < 0.01%), of which 18 were validated (p < 0.01) using RT-qPCR in an extended cohort. Compared with OSE, miR-205-5p was the most overexpressedmiRNA in HGSC. miR-200 family members and miR-182-5p were the most overexpressed in HGSC and CCC compared with OSE, whereas miR-383 was the most underexpressed.miR-205-5p and miR-200 members target epithelial-mesenchymal transition (EMT)regulators, apparently being important in tumor progression. miR-509-3-5p,miR-509-5p, miR-509-3p and miR-510 were among the strongest differentiatorsbetween HGSC and CCC, all being significantly overexpressed in CCC compared with HGSC. High miR-200c-3p expression was associated with poor progression-free (p = 0.031) and overall (p = 0.026) survival in HGSC patients. Interacting miRNA andmRNA targets, including those of a TP53-related pathway presented previously,were identified in HGSC.CONCLUSIONS: Several miRNAs differentially expressed between HGSC, CCC and OSEhave been identified, suggesting a carcinogenetic role for these miRNAs. miR-200 family members, targeting EMT drivers, were mostly overexpressed in bothsubgroups, among which miR-200c-3p was associated with survival in HGSC patients.A set of miRNAs differentiates CCC from HGSC, of which miR-509-3-5p andmiR-509-5p are the strongest classifiers. Several interactions between miRNAs andmRNAs in HGSC were mapped. PMID: 24512620 |
| Expressiion Profile | Description: Expression data from high-grade serous ovarian carcinomas (HGSC), clear cell ovarian carcinomas (CCC) and ovarian surface epithelium (OSE) Organism: Homo sapiens GEO ID: GSE47841 Platform: GPL14613 Number of samples: 30 |
| Design and Sample | Cancer Type: ovarian cancer Cancer SubType: clear cell ovarian carcinoma Cell Line: N/D Experimental Design: cancer vs normal Case Sample: clear cell ovarian carcinoma Control Sample: ovarian surface epithelium Num of Case: 9 Num of Control: 9 Quantification Software: Limma Num of miRNAs: 1105 |
| Identification | Num of Up: 40 Num of Down: 76 |