| Experiment ID | EXP00270 |
| Reference | Title: Different microRNA expression levels in gastric cancer depending on Helicobacter pylori infection. Author: Chang H, Kim N, Park JH, Nam RH, Choi YJ, Lee HS, Yoon H,Shin CM, Park YS, Kim JM, Lee DH. Journal: Gut Liver. 2015 Mar;9(2):188-96. doi: 10.5009/gnl13371. Abstract: BACKGROUND/AIMS: This study was conducted to identify microRNAs (miRNAs) that aredifferentially expressed in Helicobacter pylori-infected patients with anintestinal type of gastric cancer using miRNA microarray and to confirm thecandidate miRNA expression levels.METHODS: Total RNA was extracted from the cancerous and noncancerous regions offormalin-fixed, paraffin-embedded tissues of H. pylori-positive (n=8) or H.pylori-negative (n=8) patients with an intestinal type of gastric cancer. RNAexpression was analyzed using a 3,523 miRNA profiling microarray based on theSanger miRBase. Validation analysis was performed using TaqMan miRNA assays.RESULTS: A total of 219 miRNAs in the aber-rant miRNA profiles across the miRNAmicroarray showed at least a 2-fold change differential expression in H.pylori-positive and H. pylori-negative cancer tissues. After candi-date miRNAswere selected using online miRNA databases, TaqMan miRNA assays confirmed thatthree miRNAs (miR-99b-3p, miR-564, and miR-638) were significantly increased inthree H. pylori-positive cancer tissues compared to the H. pylori-negative cancertissues. Additionally, four miRNAs (miR-204-5p, miR-338-5p, miR-375, andmiR-548c-3p) were significantly increased in H. pylori-negative cancer tissuescompared to H. pylori-positive cancer tissues.CONCLUSIONS: miRNA expression in the intestinal type of H. pyloriinfection-dependent gastric cancer suggests that different gastric can-cerpathogenesis mechanisms could exist between H. pylori-positive and H.pylori-negative gastric cancer. Additional functional studies are required. (Gut Liver, 2015;9188-196). PMID: 25167801 |
| Expressiion Profile | Description: microRNA expressions in gastric cancer Organism: Homo sapiens GEO ID: GSE54397 Platform: GPL15159 Number of samples: 32 |
| Design and Sample | Cancer Type: gastric cancer Cancer SubType: H. Pylori negative gastric cancer Cell Line: N/D Experimental Design: cancer vs normal Case Sample: H. Pylori negative gastric cancer Control Sample: normal tissue Num of Case: 8 Num of Control: 8 Quantification Software: Limma Num of miRNAs: 1205 |
| Identification | Num of Up: 26 Num of Down: 34 |