| Experiment ID | EXP00271 |
| Reference | Title: miR-638 promotes melanoma metastasis and protects melanoma cells from apoptosisand autophagy. Author: Bhattacharya A, Schmitz U, Raatz Y, Schönherr M, Kottek T, SchauerM, Franz S, Saalbach A, Anderegg U, Wolkenhauer O, SchadendorfD, Simon JC, Magin T, Vera J, Kunz M. Journal: Oncotarget. 2015 Feb 20;6(5):2966-80. Abstract: The present study identified miR-638 as one of the most significantlyoverexpressed miRNAs in metastatic lesions of melanomas compared with primarymelanomas. miR-638 enhanced the tumorigenic properties of melanoma cells in vitroand lung colonization in vivo. mRNA expression profiling identified new candidategenes including TP53INP2 as miR-638 targets, the majority of which are involvedin p53 signalling. Overexpression of TP53INP2 severely attenuated proliferativeand invasive capacity of melanoma cells which was reversed by miR-638. Depletion of miR-638 stimulated expression of p53 and p53 downstream target genes andinduced apoptosis and autophagy. miR-638 promoter analysis identified the miR-638target transcription factor associated protein 2α (TFAP2A/AP-2α) as a directnegative regulator of miR-638, suggestive for a double-negative regulatoryfeedback loop. Taken together, miR-638 supports melanoma progression andsuppresses p53-mediated apoptosis pathways, autophagy and expression of thetranscriptional repressor TFAP2A/AP-2α. PMID: 25650662 |
| Expressiion Profile | Description: miR-638 promotes melanoma metastasis and protects melanoma cells from apoptosis and autophagy (microRNA) Organism: Homo sapiens GEO ID: GSE54492 Platform: GPL10262 Number of samples: 25 |
| Design and Sample | Cancer Type: melanoma Cancer SubType: N/D Cell Line: N/D Experimental Design: metastasis Case Sample: metastatic melanoma Control Sample: primary cancer Num of Case: 8 Num of Control: 8 Quantification Software: Limma Num of miRNAs: 661 |
| Identification | Num of Up: 6 Num of Down: 61 |